Boonpangrak Somchai, Whitcombe Michael J, Prachayasittikul Virapong, Mosbach Klaus, Ye Lei
Pure and Applied Biochemistry, Chemical Centre, Lund University, 22100 Lund, Sweden.
Biosens Bioelectron. 2006 Sep 15;22(3):349-54. doi: 10.1016/j.bios.2006.04.014. Epub 2006 May 24.
The use of molecularly imprinted polymers (MIPs) in chemical and bioanalytical applications has been gaining in interest in recent years. Compared to their biological receptor counterparts, MIPs are easy to prepare, have long shelf stability and can be used under a variety of harsh conditions. The majority of MIPs currently used are produced by traditional free radical polymerization. One drawback with the use of standard free radical initiators is that little control can be exerted over the chemical processes that form the final imprinted cavities. In this study we set out to investigate the application of controlled (living) free radical polymerization to the preparation of MIPs. This was exemplified by the synthesis of cholesterol-imprinted bulk polymers by nitroxide-mediated polymerization (NMP). A sacrificial covalent bond was employed to maintain imprinting fidelity at elevated temperature. Selective uptake of cholesterol from solutions in hexane was studied with imprinted polymers prepared under different conditions. The imprinted hydrolyzed MIP prepared by NMP displayed higher selective cholesterol binding than that prepared by a traditional radical polymerization.
近年来，分子印迹聚合物（MIPs）在化学和生物分析应用中的使用越来越受到关注。与它们的生物受体对应物相比，MIPs易于制备，具有长期的储存稳定性，并且可以在各种苛刻条件下使用。目前使用的大多数MIPs是通过传统自由基聚合生产的。使用标准自由基引发剂的一个缺点是，对形成最终印迹腔的化学过程几乎无法控制。在本研究中，我们着手研究可控（活性）自由基聚合在MIPs制备中的应用。以通过氮氧自由基介导的聚合（NMP）合成胆固醇印迹本体聚合物为例进行了说明。采用牺牲共价键在高温下保持印迹保真度。研究了用不同条件下制备的印迹聚合物从己烷溶液中选择性摄取胆固醇的情况。通过NMP制备的印迹水解MIP比通过传统自由基聚合制备的MIP表现出更高的胆固醇选择性结合。
