Chen Lei, Lin Li, Zhang Chun-zhi, Han Yun-mei
Key Laboratory of Occupational Health and Environmental Medicine, Jining Medical College, Jining 272013, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2009 Jun;27(6):321-4.
To study the combined effect of low temperature and vibration on function of peripheral circulation and nerve.
64 rabbits were divided into control group, low temperature group, vibration group and combined effect group randomly, 16 each group. The changes of concentration of ET, Ang II, NO in plasma and SCV, amplitude of sensory nerve action potential, latency of sensory nerve action potential, MCV, distal amplitude of motor nerve, and distal latency of motor nerve were measured before and after experiment.
After experiment, the concentration of ET, Ang II, NO and SCV, amplitude of sensory nerve action potential, latency of sensory nerve action potential, MCV, distal amplitude of motor nerve, and distal latency of motor nerve were (68.84+/-14.81) pg/ml, (544.01+/-70.20) pg/ml, (123.73+/-9.58) nmol/ml, (25.36+/-6.96) m/s, (1.84+/-0.65) microV, (4.05+/-1.04) m/s, (27.40+/-6.05) m/s, (1.60+/-0.52) microV, (3.51+/-1.30) m/s respectively in low temperature group; (70.22+/-15.02) pg/ml, (540.77+/-68.25) pg/ml, (129.46+/-11.99) nmol/ml, (27.69+/-6.16) m/s, (2.19+/-0.53) microV, (3.86+/-0.89) m/s, (30.03+/-5.21) m/s, (1.65+/-0.49) microV, (3.36+/-l.11) m/s respectively in vibration group; (88.47+/-13.20) pg/ml, (687.38+/-101.44) pg/ml, (70.66+/-4.99) nmol/ml, (20.82+/-3.65) m/s, (1.21+/-0.64) microV, (5.05+/-0.94) m/s, (19.97+/-4.37) m/s, (1.09+/-0.49) microV, (4.49+/-1.26) m/s respectively in combined effect group; compared with pre-experiment, the concentration of ET and Ang II in low temperature group, vibration group and combined effect group were increased after experiment, and the NO was decreased (P<0.05); the nerve conduct velocity and amplitude was decreased and the latency was delayed (P<0.05). After experiment, the concentrations of ET and Ang II in combined effect group were higher than low temperature group and vibration group, and the concentration of NO in combined effect group was lower than low temperature group and vibration group (P<0.05). After experiment, the SCV and MCV in combined effect group were slower than low temperature group and vibration group; the amplitude of sensory nerve action potential and distal amplitude of motor nerve were less than low temperature group and vibration group; the latency of sensory nerve action potential and distal latency of motor nerve in combined effect group was longer than low temperature group and vibration group. The factorial analysis results indicated the synergistic effect between low temperature and vibration (P<0.05).
Vibration-induced peripheral vascular impairment and nerve impairment would be intensified by low temperature.
研究低温与振动对周围循环及神经功能的联合影响。
将64只家兔随机分为对照组、低温组、振动组和联合作用组，每组16只。分别于实验前后测定血浆中内皮素（ET）、血管紧张素Ⅱ（AngⅡ）、一氧化氮（NO）的浓度以及感觉神经传导速度（SCV）、感觉神经动作电位幅度、感觉神经动作电位潜伏期、运动神经传导速度（MCV）、运动神经远端波幅和运动神经远端潜伏期。
实验后，低温组ET、AngⅡ、NO浓度及SCV、感觉神经动作电位幅度、感觉神经动作电位潜伏期、MCV、运动神经远端波幅、运动神经远端潜伏期分别为（68.84±14.81）pg/ml、（544.01±70.20）pg/ml、（123.73±9.58）nmol/ml、（25.36±6.96）m/s、（1.84±0.65）μV、（4.05±1.04）m/s、（27.40±6.05）m/s、（1.60±0.52）μV、（3.51±1.30）m/s；振动组分别为（70.22±15.02）pg/ml、（540.77±68.25）pg/ml、（129.46±11.99）nmol/ml、（27.69±6.16）m/s、（2.19±0.53）μV、（3.86±0.89）m/s、（30.03±5.21）m/s、（1.65±0.49）μV、（3.36±1.11）m/s；联合作用组分别为（88.47±13.20）pg/ml、（687.38±101.44）pg/ml、（70.66±4.99）nmol/ml、（20.82±3.65）m/s、（1.21±0.64）μV、（5.05±0.94）m/s、（19.97±4.37）m/s、（1.09±0.49）μV、（4.49±1.26）m/s。与实验前比较，低温组、振动组和联合作用组实验后ET、AngⅡ浓度升高，NO浓度降低（P<0.05）；神经传导速度和波幅降低，潜伏期延长（P<0.05）。实验后，联合作用组ET、AngⅡ浓度高于低温组和振动组，联合作用组NO浓度低于低温组和振动组（P<0.05）。实验后，联合作用组SCV、MCV慢于低温组和振动组；感觉神经动作电位幅度和运动神经远端波幅小于低温组和振动组；感觉神经动作电位潜伏期和运动神经远端潜伏期长于低温组和振动组。析因分析结果显示低温与振动之间存在协同作用（P<0.05）。
低温可加剧振动所致的周围血管和神经损伤。
