Jiang Jun-qiang, Ding Yi, Li Xiao-yu, Cai Wei, Wang Zhong-chao
Dept. of Oral Medicine, Stomatological Hospital of Luzhou Medical College, Luzhou 646000, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2009 Oct;27(5):538-41.
To evaluate the biological effects of Drynaria fortunei naringin on the proliferation and alkaline phosphatase (ALP) activity of human periodontal ligament cells (hPDLC).
hPDLC were primarily cultured and identified in vitro through enzyme digestion combined tissue culture method. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method was used to observe the proliferation of hPDLC with the effects of different concentrations of Drynaria fortunei naringin at difference times. The method recommended by International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) was adopted to investigate the effect of Drynaria fortune naringin on the alkaline phosphatase (ALP) activity of hPDLC. At the same time, we used bright blue method to detect the contents of protein in each sample. Then ALP activity in per milligram protein was accounted.
Significant proliferative promotion to hPDLC by Drynaria fortunei naringin could be observed at the dose of 10, 1, 0.1 mg x L(-1). Significant ALP activity of hPDLC promotion of Drynaria fortune naringin could be observed at the dose of 100, 10, 1, 0.1 mg x L(-1) and the dose of 1 mg x L(-1) Drynaria fortune naringin had greatest promotion on ALP activity of hPDLC.
Drynaria fortune naringin might significantly promote the proliferation and increase the ALP activity of hPDLC.
评价骨碎补柚皮苷对人牙周膜细胞（hPDLC）增殖及碱性磷酸酶（ALP）活性的生物学作用。
采用酶消化联合组织培养法体外原代培养并鉴定hPDLC。采用噻唑蓝（MTT）法观察不同浓度骨碎补柚皮苷在不同时间作用下hPDLC的增殖情况。采用国际临床化学和检验医学联合会（IFCC）推荐的方法研究骨碎补柚皮苷对hPDLC碱性磷酸酶（ALP）活性的影响。同时，采用考马斯亮蓝法检测各样本中的蛋白质含量。然后计算每毫克蛋白质中的ALP活性。
在10、1、0.1mg·L⁻¹剂量下可观察到骨碎补柚皮苷对hPDLC有显著的增殖促进作用。在100、10、1、0.1mg·L⁻¹剂量下可观察到骨碎补柚皮苷对hPDLC的ALP活性有显著促进作用，且1mg·L⁻¹骨碎补柚皮苷对hPDLC的ALP活性促进作用最大。
骨碎补柚皮苷可能显著促进hPDLC的增殖并提高其ALP活性。
