Jiang Jun-Qiang, Cai Wei, Wang Zhong-Chao, Ding Yi, Li Xiao-Yu
Dept. of Endodontics, Stomatological Hospital of Luzhou Medical College, Luzhou 646000, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2010 Jun;28(3):330-3.
To evaluate the effects of Drynaria fortunei naringin on the total protein content and ultra-structure of human periodontal ligament cells (hPDLCs).
Through enzyme digestion combined tissue-culture method, primarily culture and identify the human periodontal ligament cells. Coomassie brilliant blue staining was used to detect the total protein content of hPDLCs with the effects of difference concentration of Drynaria fortunei naringin at difference times. Transmission electron microscope was used to study the ultra-structure of hPDLCs with the effects of Drynaria fortunei naringin.
In vitro, the addition of Drynaria fortunei naringin at dose of 100, 10, 1, 0.1 mg x L(-1) in cultures resulted in an increase of total protein content at the 3rd, 5th, 7th day, but the maximum response was obtained with 1 mg x L(-1) Drynaria fortunei naringin. There were more rough endoplasmic reticulums, mitochodrias and ribosomes in the experimental group than in the control.
Drynaria fortunei naringin may stimulate the protein synthesis and metabolism of hPDLCs.
评估骨碎补柚皮苷对人牙周膜细胞（hPDLCs）总蛋白含量及超微结构的影响。
采用酶消化联合组织培养法，对人牙周膜细胞进行原代培养及鉴定。采用考马斯亮蓝染色法检测不同浓度骨碎补柚皮苷在不同时间作用下hPDLCs的总蛋白含量。采用透射电子显微镜观察骨碎补柚皮苷作用下hPDLCs的超微结构。
体外实验中，在培养物中添加100、10、1、0.1 mg·L⁻¹剂量的骨碎补柚皮苷，在第3、5、7天可使总蛋白含量增加，但在1 mg·L⁻¹骨碎补柚皮苷作用下获得最大反应。实验组的粗面内质网、线粒体和核糖体比对照组更多。
骨碎补柚皮苷可能刺激hPDLCs的蛋白质合成和代谢。
