D'Elia Tom, Veerapaneni Ram, Theraisnathan Vincent, Rogers Scott O
Department of Biological Sciences, Bowling Green State University, Bowling Green, Ohio 43403, USA.
Mycologia. 2009 Nov-Dec;101(6):751-63. doi: 10.3852/08-184.
Here we report the characterization of fungi from 10 accretion ice sections (3300-5100 y old) as well as two deep glacial ice sections that are close to the bottom of the glacier (1,000,000-2,000,000 y old) from the Vostok, Antarctica, 5G ice core. Fungi were characterized by fluorescence microscopy culturing and sequence analyses of ribosomal DNA internal transcribed spacers. A total of 270 fungal colonies were cultured from the accretion ice of subglacial Lake Vostok and an additional 14 from the glacial ice immediately above the accretion ice. Mean concentrations were 0-4.42 cells mL(-1) ice meltwater of which 0-100% exhibited viability (as determined by fluorescence microscopy). Thirty-one unique fungal ribosomal DNA sequences (28 from accretion ice and three from glacial ice) were determined and compared to recent taxa. The results, plus tests for growth at low temperatures, indicated that Lake Vostok contains a mixture of heterotrophic psychrotolerant fungal species. This indicates that the lake is not sterile but contains a unique ecosystem.
在此，我们报告了对来自南极洲沃斯托克5G冰芯10个堆积冰段（距今3300 - 5100年）以及靠近冰川底部的两个深层冰川冰段（距今100万 - 200万年）中的真菌的特征描述。通过荧光显微镜培养以及核糖体DNA内转录间隔区的序列分析对真菌进行了特征描述。从沃斯托克冰下湖的堆积冰中共培养出270个真菌菌落，在堆积冰正上方的冰川冰中又额外培养出14个。平均浓度为0 - 4.42个细胞/毫升融冰水，其中0 - 100%具有活力（通过荧光显微镜确定）。确定了31个独特的真菌核糖体DNA序列（28个来自堆积冰，3个来自冰川冰），并与近期的分类单元进行了比较。结果以及低温生长测试表明，沃斯托克湖含有多种异养嗜冷真菌物种。这表明该湖并非无菌，而是包含一个独特的生态系统。
