多形性胶质母细胞瘤中血管平滑肌细胞的增殖。

Proliferation of vascular smooth muscle cells in glioblastoma multiforme.

机构信息

Department of Neurosurgery, Faculty of Medical Sciences, University of Fukui, Japan.

出版信息

J Neurosurg. 2010 Aug;113(2):218-24. doi: 10.3171/2009.10.JNS08631.

DOI:10.3171/2009.10.JNS08631

PMID:19929197

Abstract

OBJECT

Glioblastomas multiforme (GBM) contain a higher number of alpha-smooth muscle actin (SMA)-positive vascular smooth muscle cells (VSMCs) than those in the respective normal neuronal tissue. The role of VSMCs during angiogenesis is unclear, and it is also uncertain whether and to what extent angiogenic factors might be involved in GBM VSMCs. In GBMs, the contribution of VSMCs in angiogenesis accompanying endothelial proliferation and the correlation of VSMC proliferation with vascular endothelial growth factor (VEGF) expression were examined using an immunohistochemical method.

METHODS

The examined material, including surrounding brain tissue, came from 12 cases (6 men and 6 women) with classic GBM. Microvessel densities (MVDs) of CD31-immunoreactive vessels (CD31-MVD) and SMA-immunoreactive vessels (SMA-MVD) were obtained in areas selected from white matter, boundary, tumor (concentrated area of tumor cells), and perinecrosis. Subsequently, the SMA-MVD/CD31-MVD (SMA/CD31) rate, representing the percentage of vessels with VSMCs in the region, was calculated in each area. The VEGF immunoreactivity of tumor cells was examined, and cases were divided into 2 groups: < 30% VEGF expression of tumor cells (low VEGF group) and > 30% VEGF expression of tumor cells (high VEGF group).

RESULTS

The SMA/CD31 rate of the boundary was significantly lower than that of the tumor (p < 0.005) and perinecrosis (p < 0.001). The SMA/CD31 rate of the high VEGF group was significantly higher than that of the low VEGF group (p < 0.05) in the tumor.

CONCLUSIONS

In GBMs, the transformation and proliferation of VSMCs may accompany neovascularization and may also be induced by angiogenic factors.

摘要

目的

多形性胶质母细胞瘤（GBM）中含有比相应正常神经组织更多的α-平滑肌肌动蛋白（SMA）阳性血管平滑肌细胞（VSMC）。在血管生成过程中 VSMC 的作用尚不清楚，也不确定血管生成因子是否以及在何种程度上参与 GBM VSMC。在 GBM 中，通过免疫组织化学方法检查 VSMC 在伴随内皮细胞增殖的血管生成中的作用，以及 VSMC 增殖与血管内皮生长因子（VEGF）表达的相关性。

方法

检查的材料包括来自 12 例（6 名男性和 6 名女性）经典 GBM 的周围脑组织。在选择的白质、边界、肿瘤（肿瘤细胞集中区）和坏死周围区获得 CD31 免疫反应性血管（CD31-MVD）和 SMA 免疫反应性血管（SMA-MVD）的微血管密度（MVD）。随后，在每个区域计算 SMA-MVD/CD31-MVD（SMA/CD31）率，代表该区域具有 VSMC 的血管百分比。检查肿瘤细胞的 VEGF 免疫反应性，并将病例分为 2 组：肿瘤细胞的 VEGF 表达<30%（低 VEGF 组）和肿瘤细胞的 VEGF 表达>30%（高 VEGF 组）。