Yeatman T J, Kimura A K, Copeland E M, Bland K I
Department of Surgery, JHMHC, University of Florida, College of Medicine, Gainesville 32610.
Ann Surg. 1991 Feb;213(2):113-7. doi: 10.1097/00000658-199102000-00003.
Recently it was found that immunoanalysis of carcinoembryonic antigen (CEA) levels in gallbladder bile may be a sensitive method to detect colorectal liver metastases in humans. Methods used in the past for the detection of CEA in various body fluids were cumbersome and time consuming, requiring acid extraction, extensive dialysis, and column purification. Single-step, solid-phase radioimmunoassays, designed specifically for serum CEA analysis, were developed commercially to replace these methods. Parameters and methodology necessary to adapt these kits for Parameters and methodology necessary to adapt these kits for use with gallbladder bile are presented here. A combination of pretreatment procedures for bile, before radioimmunoassay, permit rapid, reproducible, and accurate measurement of CEA levels in gallbladder bile.
最近发现，检测胆囊胆汁中癌胚抗原（CEA）水平的免疫分析可能是检测人类结直肠癌肝转移的一种敏感方法。过去用于检测各种体液中CEA的方法既繁琐又耗时，需要酸提取、大量透析和柱纯化。专门为血清CEA分析设计的单步固相放射免疫测定法已在商业上开发出来以取代这些方法。本文介绍了使这些试剂盒适用于胆囊胆汁检测所需的参数和方法。在放射免疫测定之前，对胆汁进行预处理程序的组合，能够快速、可重复且准确地测量胆囊胆汁中的CEA水平。
