San Luis Potosi University, Mexico, San Luis Potosi, Mexico.
J Clin Periodontol. 2009 Dec;36(12):1004-10. doi: 10.1111/j.1600-051X.2009.01496.x.
To identify periodontal bacterial DNA (PBDNA) by PCR in subgingival dental plaque (SDP), serum and synovial fluid (SF) of rheumatoid arthritis (RA) with periodontal disease (PD) patients and to explore the possible PBDNA transport pathways from mouth to joints.
This cross-sectional prolective study involved 19 subjects with RA and PD. Informed consent, health and dental questionnaires were obtained. SDP, SF and serum samples were obtained, and leucocytes were isolated from blood. DNA was extracted and PCR assays to detect main PD species were carried out. Cultures on agar plates and broth, from each sample, were performed.
Hundred percentage of patients showed PBDNA in SDP and SF and 83.5% in serum. Prevotella intermedia (89.4% and 73.6%) and Porphyromonas gingivalis (57.8% and 42.1%) were the species most frequently detected in SDP and SF, respectively. In SDP, 4.05 different bacterial species were found followed by 1.19 in serum and 2.26 in SF. Culture onto agar plates and broth did not show any bacterial growth, leucocytes were not positive to PBDNA by PCR.
This study suggests that PBDNA could have a role on the RA aetiology. The possible pathway of transport of PBDNA from mouth to joints could be via the free form of DNA.
通过聚合酶链反应（PCR）检测伴牙周病类风湿关节炎（RA）患者龈下牙菌斑（SDP）、血清和滑液（SF）中的牙周细菌 DNA（PBDNA），探讨 PBDNA 从口腔到关节的可能传输途径。
本横断面前瞻性研究纳入 19 例伴 PD 的 RA 患者。获得知情同意、健康和牙科问卷后，采集 SDP、SF 和血清样本，并从血液中分离白细胞。提取 DNA 后，采用 PCR 检测主要 PD 种属。对每个样本进行琼脂平板和肉汤培养。
100%的患者在 SDP 和 SF 中以及 83.5%的患者在血清中均显示出 PBDNA。中间普氏菌（89.4%和 73.6%）和牙龈卟啉单胞菌（57.8%和 42.1%）分别是 SDP 和 SF 中最常检测到的种属。在 SDP 中发现了 4.05 种不同的细菌，血清中为 1.19 种，SF 中为 2.26 种。琼脂平板和肉汤培养均未显示任何细菌生长，PCR 也未显示白细胞中存在 PBDNA。
本研究表明 PBDNA 可能在 RA 发病机制中起作用。PBDNA 从口腔到关节的可能传输途径可能是游离 DNA 形式。
