Britos Marcelo, Hernández Marcela, Fernández Alejandra, Pellegrini Elizabeth, Chaparro Laura, Chaparro Alejandra, Suárez Lina J, Hoare Anilei, Hernández-Ríos Patricia
Laboratory of Periodontal Biology, Faculty of Dentistry, Universidad de Chile, Olivos 943, Independencia, Santiago, 8380544, Chile.
Department of Pathology and Oral Medicine, Faculty of Dentistry, Universidad de Chile, Olivos 943, Independencia, Santiago, 8380544, Chile.
Clin Oral Investig. 2025 May 7;29(6):288. doi: 10.1007/s00784-025-06363-9.
To determine bacterial blood translocation signatures and their association with the subgingival microbiota in individuals with and without periodontitis.
Cross-sectional study. DNA was extracted from blood and subgingival samples of individuals with periodontitis (n = 21) and control volunteers (n = 24). Subgingival microbiota was explored by 16 S rRNA gene sequencing. Detection frequency and loads of total bacteria, Porphyromonas gingivalis (Pg), Porphyromonas endodontalis (Pe) and Fusobacterium nucleatum (Fn) were determined in all samples using quantitative polymerase chain reaction (qPCR). The statistical analysis was performed using STATA 16.
Subgingival samples from individuals with periodontitis presented higher relative abundance of Prevotella intermedia, F. nucleatum subsp. vincentii, Treponema sp. HMT 237, Alloprevotella tannerae, Filifactor alocis, Pg, Treponema denticola and Pe, and higher loads of total bacteria, Pg, Pe and Fn, compared to the control group (p < 0.001). While Pg and Fn were not detected in blood, Pe was detected in 95% of individuals with periodontitis and 83% of the control ones (p = 0.205), with higher loads in blood samples from periodontitis (p = 0.034). No significant correlation was found between subgingival bacterial loads and blood loads of Pe in periodontitis and control groups (p > 0.05).
Individuals with periodontitis presented higher relative abundance and loads of periodontal bacteria in subgingival samples and higher Pe loads in blood samples, although further research is needed to understand the correlation between subgingival and blood bacterial loads.
The present study showed higher loads of Pe in the blood of individuals with periodontitis, suggesting potential extraoral dissemination and a linking mechanism with several systemic diseases.
确定患有和未患牙周炎个体的细菌血行转移特征及其与龈下微生物群的关联。
横断面研究。从患有牙周炎的个体（n = 21）和对照志愿者（n = 24）的血液和龈下样本中提取DNA。通过16S rRNA基因测序探究龈下微生物群。使用定量聚合酶链反应（qPCR）测定所有样本中总细菌、牙龈卟啉单胞菌（Pg）、牙髓卟啉单胞菌（Pe）和具核梭杆菌（Fn）的检测频率和载量。使用STATA 16进行统计分析。
与对照组相比，患有牙周炎个体的龈下样本中中间普氏菌、具核梭杆菌文森亚种、梅毒螺旋体属HMT 237、坦纳氏异普雷沃菌、纤细杆菌、Pg、齿垢密螺旋体和Pe的相对丰度更高，总细菌、Pg、Pe和Fn的载量也更高（p < 0.001）。虽然血液中未检测到Pg和Fn，但在95%的牙周炎患者和83%的对照者中检测到了Pe（p = 0.205），牙周炎患者血液样本中的载量更高（p = 0.034）。在牙周炎组和对照组中，龈下细菌载量与血液中Pe载量之间未发现显著相关性（p > 0.05）。
患有牙周炎的个体龈下样本中牙周细菌的相对丰度和载量更高，血液样本中Pe载量更高，尽管需要进一步研究以了解龈下和血液细菌载量之间的相关性。
本研究显示牙周炎患者血液中Pe载量更高，提示可能存在口外播散以及与多种全身性疾病的联系机制。
