Department of Esophageal and Gastroenterological Surgery, Juntendo University, School of Medicine, Bunkyo-ku, Tokyo, Japan.
Dis Esophagus. 2010 Jul;23(5):415-21. doi: 10.1111/j.1442-2050.2009.01026.x. Epub 2009 Nov 23.
Squamous cell carcinoma of the esophagus (ESCC) has a poor prognosis among digestive tract cancers. Lymph node metastasis and distant metastasis are the major factors determining its prognosis. We used comparative genomic hybridization (CGH) to evaluate primary tumor lymph nodes and metastatic areas from ESCC patients in order to determine the relationship between abnormal chromosome regions and outcome. Tumor tissues and lymph nodes were collected from 51 patients with ESCC, and abnormal chromosome regions were detected by CGH. We searched for regions that were significantly more common in patients with lymph nodes metastases (n>/= 6) or distant metastases, and correlated those chromosomal changes with survival. Regions showing amplification in more than 65% of esophageal squamous cell cancers were as follows: 17q12 (90.2%), 17q21 (86.3%), 3q29 (82.4%), 3q28 (78.4%), 8q24.2 (76.5%), 22q12 (76.5%), 3q27 (74.5%), 8q24.3 (74.5%), 1q22 (70.6%), 5p15.3 (70.6%), 22q13 (70.6%), 3q26.3, 8q23, 8q24.1, 9q34, 11q13, 17p12, 17q25, 20q12, 20q13.1 (68.6%), 1q32, 1q42, and 20q13.2 (66.7%). Regions showing deletion in more than 50% of the tumors were as follows: Yp11.3 (62.7%), 3p26 (56.9%), Yq12 (54.9%), 13q21 (52.9%), 4q32 (51.0%), and 13q22 (51.0%). When Fisher's test was used to assess associations of these regions with metastases to lymph nodes, amplification at 2q12-14 (P= 0.012), 3q24-26 (P= 0.005), and 7q21-31 (P= 0.026) were significant. Survival was worse for patients with amplification at all 3 regions. In patients with distant organ metastases, amplification at 7p13-21 was significant (P= 0.008), and survival was worse. Chromosomal amplifications in ESCC at 2q12-14, 3q24-26, and 7q21-31 were associated with lymph node metastasis, while amplification at 7p13-21 was related to distant metastasis. Amplification at these regions correlated with worse survival. Genes involved in the phenotype of ESCC may exist in these regions. Identification of these genes is a theme for future investigation.
食管鳞状细胞癌（ESCC）在消化道癌症中预后较差。淋巴结转移和远处转移是决定其预后的主要因素。我们使用比较基因组杂交（CGH）来评估 ESCC 患者的原发肿瘤淋巴结和转移区域，以确定异常染色体区域与结果之间的关系。从 51 名 ESCC 患者中收集肿瘤组织和淋巴结，并通过 CGH 检测异常染色体区域。我们寻找在淋巴结转移（n>/=6）或远处转移患者中更常见的区域，并将这些染色体变化与生存相关联。在超过 65%的食管鳞状细胞癌中显示扩增的区域如下：17q12（90.2%）、17q21（86.3%）、3q29（82.4%）、3q28（78.4%）、8q24.2（76.5%）、22q12（76.5%）、3q27（74.5%）、8q24.3（74.5%）、1q22（70.6%）、5p15.3（70.6%）、22q13（70.6%）、3q26.3、8q23、8q24.1、9q34、11q13、17p12、17q25、20q12、20q13.1（68.6%）、1q32、1q42 和 20q13.2（66.7%）。在超过 50%的肿瘤中显示缺失的区域如下：Yp11.3（62.7%）、3p26（56.9%）、Yq12（54.9%）、13q21（52.9%）、4q32（51.0%）和 13q22（51.0%）。当使用 Fisher 检验评估这些区域与淋巴结转移的关联时，2q12-14（P=0.012）、3q24-26（P=0.005）和 7q21-31（P=0.026）的扩增具有显著意义。所有 3 个区域均出现扩增的患者的生存状况更差。在发生远处器官转移的患者中，7p13-21 处的扩增具有显著意义（P=0.008），且生存状况更差。ESCC 中 2q12-14、3q24-26 和 7q21-31 处的染色体扩增与淋巴结转移相关，而 7p13-21 处的扩增与远处转移相关。这些区域的扩增与生存状况较差相关。在这些区域可能存在与 ESCC 表型相关的基因。鉴定这些基因是未来研究的主题。
