Plesnicar Andrej, Vidmar G, Stabuc B, Kores Plesnicar B
University College of Health Studies, University of Ljubljana, Ljubljana, Slovenia.
J Int Med Res. 2009 Sep-Oct;37(5):1570-6. doi: 10.1177/147323000903700535.
Multiple myeloma (MM) remains largely incurable, although traditional chemotherapy and new compounds have been shown to produce a clinical response. Clinical studies were performed to determine the effectiveness of interferon-alpha (IFN-alpha) in MM, which has also recently been shown to function as a survival factor for MM cells. The effects of different doses of native human leukocyte interferon-alpha (nhIFN-alpha), recombinant human interferon-alpha2a (rhIFN-alpha2a) and recombinant human interferon-alpha2b (rhIFN-alpha2b) on in vitro P3-X63-Ag8.653 mouse myeloma cell growth were compared. A statistically significant dose-dependent reduction in cell viability following cell culture with nhIFN-alpha was observed. On the other hand, a statistically significant increase in cell viability was observed following cell culture with rhIFN-alpha2a and rhIFN-alpha2b, but only in relation to the control group and seemingly without dose dependency. These results highlight the importance of the type of human IFN-alpha used in the treatment and study of MM, and suggest that nhIFN-alpha may have a role in future personalized therapy approaches.
多发性骨髓瘤（MM）在很大程度上仍然无法治愈，尽管传统化疗和新化合物已显示出能产生临床反应。开展了临床研究以确定α干扰素（IFN-α）在MM中的有效性，最近还发现它可作为MM细胞的生存因子发挥作用。比较了不同剂量的天然人白细胞α干扰素（nhIFN-α）、重组人α干扰素2a（rhIFN-α2a）和重组人α干扰素2b（rhIFN-α2b）对体外培养的P3-X63-Ag8.653小鼠骨髓瘤细胞生长的影响。观察到用nhIFN-α进行细胞培养后，细胞活力出现了具有统计学意义的剂量依赖性降低。另一方面，在用rhIFN-α2a和rhIFN-α2b进行细胞培养后，观察到细胞活力有统计学意义的增加，但仅相对于对照组而言，且似乎无剂量依赖性。这些结果突出了用于MM治疗和研究的人IFN-α类型的重要性，并表明nhIFN-α可能在未来的个性化治疗方法中发挥作用。
