P3-X63-Ag8和X63-Ag8.653小鼠骨髓瘤细胞体外生长的胆固醇需求
Cholesterol requirement of P3-X63-Ag8 and X63-Ag8.653 mouse myeloma cells for growth in vitro.
作者信息
Sato J D, Kawamoto T, Okamoto T
出版信息
J Exp Med. 1987 Jun 1;165(6):1761-6. doi: 10.1084/jem.165.6.1761.
Abstract
P3-X63-Ag8 and X63-Ag8.653 mouse myeloma cells have an absolute requirement for cholesterol for growth under serum-free conditions. This requirement can be satisfied by low density lipoprotein at 2-6 micrograms/ml or by BSA-bound cholesterol at 5-10 micrograms/ml. Cholesterol-independent variants can be selected after prolonged growth in low concentrations of serum.
摘要
P3-X63-Ag8和X63-Ag8.653小鼠骨髓瘤细胞在无血清条件下生长时对胆固醇有绝对需求。这种需求可通过2-6微克/毫升的低密度脂蛋白或5-10微克/毫升的牛血清白蛋白结合胆固醇来满足。在低浓度血清中长时间培养后可筛选出不依赖胆固醇的变体。
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