转运RNA动力学的实验与计算测定

Experimental and computational determination of tRNA dynamics.

作者信息

Alexander Rebecca W, Eargle John, Luthey-Schulten Zaida

机构信息

Department of Chemistry, Wake Forest University, Winston-Salem, NC 27109-7486, United States.

出版信息

FEBS Lett. 2010 Jan 21;584(2):376-86. doi: 10.1016/j.febslet.2009.11.061.

DOI:10.1016/j.febslet.2009.11.061

PMID:19932098

Abstract

As the molecular representation of the genetic code, tRNA plays a central role in the translational machinery where it interacts with several proteins and other RNAs during the course of protein synthesis. These interactions exploit the dynamic flexibility of tRNA. In this minireview, we discuss the effects of modified bases, ions, and proteins on tRNA structure and dynamics and the challenges of observing its motions over the cycle of translation.

摘要

作为遗传密码的分子表现形式，转运RNA（tRNA）在翻译机制中发挥着核心作用，在蛋白质合成过程中它会与多种蛋白质和其他RNA相互作用。这些相互作用利用了tRNA的动态灵活性。在这篇小型综述中，我们讨论了修饰碱基、离子和蛋白质对tRNA结构与动态的影响，以及在翻译循环过程中观察其运动所面临的挑战。