Strobel G, Weicker H
Department of Pathophysiology and Sports Medicine, University of Heidelberg, F.R.G.
Clin Chem. 1991 Feb;37(2):196-9.
A method is described to measure catecholamine sulfates from human plasma and urine by isocratic reversed-phase high-performance liquid chromatography with electrochemical detection. For this measurement we use catecholamine 3-sulfate isomers as internal standards and determine the sulfoconjugates only after eliminating the catecholamines. Catecholamines that have previously been used as internal standards are shown to cause a significant overestimation (P less than 0.05) of the catecholamine sulfates--by 10% to 25% and 20% to 42% in human plasma and urine, respectively. The detection limits (signal-to-noise ratio greater than 3) in plasma and urine samples were about 80 pmol/L for each analyte. The intra-assay and interassay CVs were less than 4.0% and 10.6% in human plasma and less than 6.6% and 12.8% in human urine, respectively. The calibration curves for all catecholamine sulfates in human plasma and urine were linear (r greater than 0.96; P less than 0.001) over the respective concentration ranges of 0.1-100 nmol/L and 5-1000 nmol/L.
描述了一种通过等度反相高效液相色谱结合电化学检测来测量人血浆和尿液中儿茶酚胺硫酸盐的方法。对于该测量，我们使用儿茶酚胺3 - 硫酸盐异构体作为内标，并且仅在去除儿茶酚胺后测定硫酸共轭物。已被用作内标的儿茶酚胺被证明会导致儿茶酚胺硫酸盐的显著高估（P小于0.05）——在人血浆和尿液中分别高估10%至25%和20%至42%。血浆和尿液样本中的检测限（信噪比大于3）对于每种分析物约为80 pmol/L。人血浆中的批内和批间CV分别小于4.0%和10.6%，人尿液中的批内和批间CV分别小于6.6%和12.8%。人血浆和尿液中所有儿茶酚胺硫酸盐的校准曲线在各自0.1 - 100 nmol/L和5 - 1000 nmol/L的浓度范围内呈线性（r大于0.96；P小于0.001）。
