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新型花菁染料超分子组装体对特定 G-四链体结构的验证：II. 与特定的分子内 G-四链体的结合特性和识别机制。

Verification of specific G-quadruplex structure by using a novel cyanine dye supramolecular assembly: II. The binding characterization with specific intramolecular G-quadruplex and the recognizing mechanism.

机构信息

Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.

出版信息

Nucleic Acids Res. 2010 Jan;38(3):1022-33. doi: 10.1093/nar/gkp1045. Epub 2009 Nov 19.

DOI:10.1093/nar/gkp1045
PMID:19933263
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2817466/
Abstract

The supramolecular assembly of a novel cyanine dye, 3,3'-di(3-sulfopropyl)-4,5,4',5'-dibenzo-9-ethyl-thiacarbocyanine triethylammonium salt (ETC) was designed to verify specific intramolecular G-quadruplexes from duplex and single-strand DNAs. Spectral results have shown that ETC presented two major distinct signatures with specific intramolecular G-quadruplexes in vitro: (i) dramatic changes in the absorption spectra (including disappearance of absorption peak around 660 nm and appearance of independent new peak around 584 nm); (ii) approximately 70 times enhancement of fluorescence signal at 600 nm. Furthermore, based on (1)H-nuclear magnetic resonance and circular dichroism results, the preferring binding of ETC to specific intramolecular G-quadruplexes probably result from end-stacking, and the loop structure nearby also plays an important role.

摘要

新型花菁染料 3,3'-二(3-磺丙基)-4,5,4',5'-二苯-9-乙基噻二碳菁三乙铵盐（ETC）的超分子组装被设计用于验证来自双链和单链 DNA 的特定分子内 G-四链体。光谱结果表明，ETC 在体外与特定的分子内 G-四链体呈现出两个主要的明显特征：（i）吸收光谱发生剧烈变化（包括 660nm 左右吸收峰的消失和 584nm 左右独立新峰的出现）；（ii）在 600nm 处荧光信号增强约 70 倍。此外，根据（1）H 核磁共振和圆二色性结果，ETC 优先与特定的分子内 G-四链体结合可能源于末端堆积，并且附近的环结构也起着重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d1a/2817466/2ec910b06c8e/gkp1045f11.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d1a/2817466/6d1008416136/gkp1045f5.jpg
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