Koob Jennifer, Jacob Fritz, Wenning Mareike, Hutzler Mathias
Technische Universität München, Forschungszentrum Weihenstephan für Brau- und Lebensmittelqualität, Alte Akademie 3, 85354 Freising, Germany.
Department Microbiology, Technische Universität München, Zentralinstitut für Ernährungs- und Lebensmittelforschung, Weihenstephaner Berg 3, 85354 Freising, Germany.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3452-3457. doi: 10.1099/ijsem.0.002139. Epub 2017 Sep 4.
A Gram-stain-positive, non-motile, rod-shaped bacterium, designated TUM BP 140423000-2250T (=DSM 100836T=LMG 29073T), was isolated from spoiled beer. This bacterium did not form spores, and was catalase-negative and facultatively anaerobic. Its taxonomic position was determined in a polyphasic study. The 16S rRNA gene sequence similarity data showed that the strain belonged to the Lactobacillus genus with the nearest neighbours being Lactobacillus koreensis DCY50T (sequence similarity 99.5 %), Lactobacillus yonginensis THK-V8T (99.2 %) and Lactobacillus parabrevis LMG 11984T (98.7 %). Sequence comparisons of additional phylogenetic markers, pheS and rpoA, confirmed the 16S rRNA gene sequence tree topology. The maximum rpoA sequence similarity was 92.3 % with L. yonginensis THK-V8T. The DNA G+C content of the isolate was 50.0 mol%. The DNA-DNA relatedness showed that strain TUM BP 140423000-2250T could be clearly distinguished from L. koreensis DCY 50T (30.8±0.4 %) and L. yonginensis THK-V8T (23.6±5.9 %). The major fatty acids were C18 : 1ω9c, summed feature 7 (comprised of C19 : 0 cyclo ω10c/C19 : 1ω6c) and C16 : 0. Based on phenotypic and genotypic studies, the authors propose classifying the new isolate as a representative of a novel species of the genus Lactobacillus, Lactobacillus cerevisiae sp. nov. The type strain is deposited at the Research Centre Weihenstephan for Brewing and Food Quality as TUM BP 140423000-2250T (=DSM 100836T=LMG 29073T).
从变质啤酒中分离出一株革兰氏阳性、无运动性、杆状细菌，命名为TUM BP 140423000 - 2250T（=DSM 100836T = LMG 29073T）。该细菌不形成芽孢，过氧化氢酶阴性，兼性厌氧。通过多相研究确定了其分类地位。16S rRNA基因序列相似性数据表明，该菌株属于乳杆菌属，最接近的亲缘种为韩国乳杆菌DCY50T（序列相似性99.5%）、龙仁乳杆菌THK - V8T（99.2%）和副短乳杆菌LMG 11984T（98.7%）。对另外的系统发育标记物pheS和rpoA进行序列比较，证实了16S rRNA基因序列树的拓扑结构。rpoA序列与龙仁乳杆菌THK - V8T的最大相似性为92.3%。该分离株的DNA G + C含量为50.0 mol%。DNA - DNA相关性表明，菌株TUM BP 140423000 - 2250T可与韩国乳杆菌DCY 50T（30.8±0.4%）和龙仁乳杆菌THK - V8T（23.6±5.9%）明显区分。主要脂肪酸为C18 : 1ω9c、总和特征7（由C19 : 0环ω10c/C19 : 1ω6c组成）和C16 : 0。基于表型和基因型研究，作者建议将该新分离株分类为乳杆菌属的一个新物种——酿酒乳杆菌的代表种。模式菌株保藏于魏恩斯泰芬酿造与食品质量研究中心，编号为TUM BP 140423000 - 2250T（=DSM 100836T = LMG 29073T）。
