Microbial Biotechnology Laboratory, Department of Microbiology, School of Biology, College of Science, University of Tehran, 14155-6455, Tehran, Iran.
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen, Inhoffenstraße 7b, 38124 Braunschweig, Germany.
Int J Syst Evol Microbiol. 2010 Oct;60(Pt 10):2346-2352. doi: 10.1099/ijs.0.018366-0. Epub 2009 Nov 20.
A polyphasic taxonomic study of a halotolerant bacterium, isolated from sandy rhizospheric soil in Sarbandar, Persian Gulf, Iran, revealed that strain HM6(T) represents a novel species within the genus Nocardiopsis. Results of the 16S rRNA gene sequence comparison revealed that strain HM6(T) clustered with strains of the genus Nocardiopsis, showing the highest degree of 16S rRNA gene sequence similarity to Nocardiopsis quinghaiensis (99.2 %), Nocardiopsis aegyptia (98.5 %) and Nocardiopsis halotolerans (98.3 %). However, DNA-DNA hybridization studies with these type strains revealed less than 39.6 % similarity. Rather than genotypic differences, there are some phenotypic discrepancies between strain HM6(T) and closely related species of the genus Nocardiopsis. Main morphological and chemotaxonomical features of strain HM6(T) include: (i) growth characteristics, i.e. the formation of a scant light-yellow to white aerial mycelium and the typical zig-zag form of the hyphae, which fragment during ageing into smooth rod-shaped spores; (ii) the presence of meso-diaminopimelic acid and glucose plus ribose in whole-cell hydrolysates; (iii) the presence of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and phosphatidylinositol, together with three unknown Nocardiopsis-specific phospholipids (close to diphosphatidylglycerol in position) in polar lipid extracts; (iv) the presence of the major menaquinones MK-10(H₀), MK-10(H₂) and MK-9(H₀) in the non-polar fraction; (v) the presence of iso/anteiso-branched plus 10-methyl-branched fatty acids, showing the diagnostic combination for species of the genus Nocardiopsis of iso-16 : 0 (31.1 %), anteiso-17 : 0 (19.2 %), 10-methyl-17 : 0 (5.8 %) and tuberculostearic acid (8.8 %); and (vi) the absence of mycolic acids. Analysis of the 16S rRNA gene sequence revealed that strain HM6(T) represents a distinct taxon within the genus Nocardiopsis. Based upon genotypic and phenotypic differences from other members of the genus, a novel species, Nocardiopsis sinuspersici sp. nov., is proposed. The type strain is HM6(T) (=UTMC 00102(T) =DSM 45277(T) =CCUG 57624(T)).
从伊朗波斯湾的 Sarbandar 的沙质根际土壤中分离到一株耐盐细菌，对其进行多相分类学研究表明，菌株 HM6(T) 代表了诺卡氏菌属中的一个新种。16S rRNA 基因序列比较结果表明，菌株 HM6(T) 与诺卡氏菌属的菌株聚类在一起，与青海诺卡氏菌 (99.2%)、埃及诺卡氏菌 (98.5%) 和耐盐诺卡氏菌 (98.3%) 的 16S rRNA 基因序列相似性最高。然而，与这些模式菌株的 DNA-DNA 杂交研究表明，相似性低于 39.6%。菌株 HM6(T) 与诺卡氏菌属的近缘种之间存在一些表型差异，而不是基因型差异。菌株 HM6(T) 的主要形态学和化学分类学特征包括：(i) 生长特性，即形成稀疏的浅黄色至白色气生菌丝体和典型的菌丝体之字形形态，随着老化，菌丝体分裂成光滑的杆状孢子；(ii) 全细胞水解物中存在 meso-二氨基庚二酸和葡萄糖加核糖；(iii) 极性脂质提取物中存在二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱和磷脂酰肌醇以及三种未知的诺卡氏菌特异性磷脂（在位置上接近二磷脂酰甘油）；(iv) 非极性部分存在主要的menaquinones MK-10(H₀)、MK-10(H₂) 和 MK-9(H₀)；(v) 存在异/正支链加 10-甲基支链脂肪酸，具有诊断性组合，表明属于诺卡氏菌属的种为 iso-16:0 (31.1%)、anteiso-17:0 (19.2%)、10-methyl-17:0 (5.8%) 和结核硬脂酸 (8.8%)；(vi) 不存在分枝菌酸。16S rRNA 基因序列分析表明，菌株 HM6(T) 代表了诺卡氏菌属中的一个独特分类群。基于与属内其他成员的基因型和表型差异，提出了一个新的种，即正弦诺卡氏菌 (Nocardiopsis sinuspersici) sp. nov.。模式菌株为 HM6(T) (=UTMC 00102(T)=DSM 45277(T)=CCUG 57624(T))。
