Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Centre of Advanced Study in Marine Biology, Faculty of Marine Science, Annamalai University, Parangipettai 608 502, Tamilnadu, India.
Int J Syst Evol Microbiol. 2012 May;62(Pt 5):1110-1116. doi: 10.1099/ijs.0.032979-0. Epub 2011 Jun 24.
In this study, the taxonomic position of an actinobacterium, strain YIM M 10931(T), which was isolated from a mangrove sediment sample collected in Dugong Creek, Little Andaman, India, was determined by a polyphasic approach. This gram-positive, aerobic strain produced branched substrate mycelium and aerial hyphae, which differentiated into short, hooked or spiral spore chains. The organism contained meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The whole cell sugars consisted of mannose, ribose, glucose, galactose and madurose. The cellular fatty acid profile mainly consisted of iso-C(16 : 0), 10-methyl C(18 : 0) and C(16 : 0). The quinone system was predominantly composed of MK-9(H(8)) (45.5 %) and MK-9(H(6)) (39 %). The phospholipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol and two unknown phospholipids. The organism showed a combination of morphological and chemotaxonomic properties typical of members of the genus Actinomadura. Moreover, phylogenetic analysis based on a 16S rRNA gene sequence generated from the strain identified its closest relatives as Actinomadura cremea DSM 43676(T) (98.4 % sequence similarity), Actinomadura rifamycini DSM 43936(T) (97.4 %) and Actinomadura apis IM17-1(T) (96.9 %). It was obvious from the resulting phylogenetic trees that strain YIM M 10931(T) belongs to a distinct subclade within the evolutionary radiation of the genus Actinomadura. DNA-DNA hybridizations of strain YIM M 10931(T) with A. cremea DSM 43676(T) and A. rifamycini DSM 43936(T) were performed and further confirmed that the isolate represents a separate genomic species. Based on the phenotypic and genotypic characteristics presented, it is proposed that strain YIM M 10931(T) represents a novel species within the genus Actinomadura, for which the name Actinomadura sediminis sp. nov. is proposed; the type strain is YIM M 10931(T) ( = CCTCC AA 2010009(T) = DSM 45500(T)).
在这项研究中，通过多相分类方法确定了一株来自印度小安达曼岛 Dugong Creek 红树林沉积物样本的放线菌菌株 YIM M 10931(T)的分类地位。该革兰氏阳性、需氧菌株产生分支的基质菌丝和气生菌丝，这些菌丝分化成短的、钩状或螺旋状的孢子链。该生物的细胞壁肽聚糖中含有特征性的二氨基庚二酸即meso-二氨基庚二酸。全细胞糖由甘露糖、核糖、葡萄糖、半乳糖和马杜糖组成。细胞脂肪酸谱主要由 iso-C(16:0)、10-甲基 C(18:0)和 C(16:0)组成。醌系统主要由 MK-9(H(8))(45.5%)和 MK-9(H(6))(39%)组成。检测到的磷脂有双磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇甘露糖苷、磷脂酰肌醇和两种未知的磷脂。该生物表现出典型的放线菌属形态和化学分类特征。此外，基于菌株的 16S rRNA 基因序列生成的系统发育分析将其最接近的亲缘关系确定为 Actinomadura cremea DSM 43676(T)(98.4%序列相似性)、Actinomadura rifamycini DSM 43936(T)(97.4%)和 Actinomadura apis IM17-1(T)(96.9%)。从生成的系统发育树可以明显看出，菌株 YIM M 10931(T)属于放线菌属进化辐射中的一个独特分支。对菌株 YIM M 10931(T)与 A. cremea DSM 43676(T)和 A. rifamycini DSM 43936(T)进行 DNA-DNA 杂交，并进一步证实该分离株代表一个独立的基因组种。基于表型和基因型特征，建议菌株 YIM M 10931(T)代表放线菌属中的一个新种，命名为 Actinomadura sediminis sp. nov.；模式菌株为 YIM M 10931(T)( = CCTCC AA 2010009(T) = DSM 45500(T))。
