Okamoto Y, Horisaka Y, Matsumoto N, Yoshimura Y, Kawada J, Yamashita K, Takagi T
Department of Removable Prosthodontics and Anatomy, School of Dentistry, Tokushima University, Japan.
Clin Orthop Relat Res. 1991 Feb(263):242-53.
Histologic changes of muscle tissue in the early stage of heterotopic osteogenesis induced by syngeneic insoluble bone matrix gelatin (BMG) with bone morphogenetic protein in rats was observed by light and electron microscopy. BMG induced cartilage in muscle tissue by Day 7 after its implantation, woven bone by Day 10, and lamellar bone with bone marrow by Day 14. The new findings in this work include (1) the disappearance of the basement membrane of muscle fibers; (2) the activation of the satellite cells of muscle fibers; (3) the appearance of fibroblastlike cells that closely resembled activated satellite cells among the degenerated muscle fibers or on the surface of the BMG; and (4) the change of fibroblastlike cells to chondroblasts or osteoblasts. These findings suggest that intramuscular implantation of BMG caused the conspicuous disappearance of the basement membrane of the muscle fiber and may play a part in osteogenesis induced by BMG.
通过光镜和电镜观察了同基因不溶性骨基质明胶（BMG）与骨形态发生蛋白诱导大鼠异位成骨早期肌肉组织的组织学变化。BMG植入后第7天在肌肉组织中诱导形成软骨，第10天诱导形成编织骨，第14天诱导形成含骨髓的板层骨。这项工作的新发现包括：（1）肌纤维基底膜消失；（2）肌纤维卫星细胞活化；（3）在退变的肌纤维中或BMG表面出现与活化卫星细胞极为相似的成纤维样细胞；（4）成纤维样细胞向软骨细胞或成骨细胞转变。这些发现提示，肌内植入BMG导致肌纤维基底膜明显消失，可能在BMG诱导的成骨过程中发挥作用。
