Yamashita K, Horisaka Y, Okamoto Y, Yoshimura Y, Matsumoto N, Kawada J, Takagi T
Department of Anatomy, School of Dentistry, University of Tokushima, Japan.
Acta Anat (Basel). 1991;141(1):1-7. doi: 10.1159/000147090.
Heterotopic bone formation induced by demineralized bone matrix gelatin (BMG) in bupivacaine-HCl-treated skeletal muscle was examined histologically. BMG was obtained by dehydrating diaphyseal shafts of femora and tibiae of male, 4-week-old Sprague-Dawley (SD) rats, cutting it into chips, and demineralizing and extracting the chips with various solutions. The BMG was implanted into the rectus abdominis muscle of male, 5-week-old SD rats, bupivacaine-HCl was injected at the same site, and the resulting plaques of tissues were examined histologically on days 5, 10, 15 and 20 after BMG implantation. Heterotopic bone formation occurred in all animals. The bupivacaine-treated group had more degenerated and injured muscle fibers, and more osteocytes than the control group. Electron microscopy showed that the basement membrane of muscle fibers was discontinuous and that many mononucleated cells resembling activated satellite cells were present on day 5. Many fibroblasts, undifferentiated mesenchymal cells and myogenic cells were seen in the area around the BMG. In new bones there were few osteocytes on day 10, but their numbers were increased on days 15 and 20 after implantation, especially in the bupivacaine-treated group. The population of osteocytes that increased rapidly may have included mononucleated cells similar to activated satellite cells.
对布比卡因 - HCl处理的骨骼肌中脱矿骨基质明胶（BMG）诱导的异位骨形成进行了组织学检查。BMG是通过将4周龄雄性Sprague - Dawley（SD）大鼠的股骨和胫骨骨干脱水，切成薄片，并用各种溶液对薄片进行脱矿和提取而获得的。将BMG植入5周龄雄性SD大鼠的腹直肌中，在同一部位注射布比卡因 - HCl，并在BMG植入后第5、10、15和20天对所得组织斑块进行组织学检查。所有动物均发生了异位骨形成。与对照组相比，布比卡因治疗组的肌纤维退变和损伤更多，骨细胞也更多。电子显微镜显示，在第5天，肌纤维的基底膜不连续，并且存在许多类似于活化卫星细胞的单核细胞。在BMG周围区域可见许多成纤维细胞、未分化的间充质细胞和生肌细胞。在新骨中，第10天时骨细胞很少，但在植入后第15天和20天其数量增加，尤其是在布比卡因治疗组。迅速增加的骨细胞群体可能包括类似于活化卫星细胞的单核细胞。
