Université de Toulouse, UPS, Centre de Recherches sur la Cognition Animale, F-31062 Toulouse Cedex 9, France.
Learn Mem. 2009 Nov 23;16(12):761-5. doi: 10.1101/lm.1603009. Print 2009 Dec.
Harnessed bees learn to associate an odorant with an electric shock so that afterward the odorant alone elicits the sting extension response (SER). We studied the dependency of retention on interstimulus interval (ISI), intertrial interval (ITI), and number of conditioning trials in the framework of olfactory SER conditioning. Forward ISIs (conditioned stimulus [CS] before unconditioned stimulus [US]) supported higher retention than a backward one (US before CS) with an optimum around 3 sec. Spaced trials (ITI 10 min) supported higher retention than massed trials (ITI 1 min) and led to the formation of a late long-term memory (l-LTM) that depended on protein synthesis. Our results reaffirm olfactory SER conditioning as a reliable tool for the study of learning and memory.
经过训练，蜜蜂学会将一种气味与电击联系起来，以至于之后仅气味本身就能引发蜇刺伸展反应（SER）。我们在嗅觉 SER 条件反射的框架内研究了保留依赖于刺激间间隔（ISI）、试验间间隔（ITI）和条件反射试验次数的情况。前向 ISI（条件刺激 [CS] 在非条件刺激 [US] 之前）比后向 ISI（US 在 CS 之前）支持更高的保留率，最佳间隔时间约为 3 秒。间隔试验（ITI 为 10 分钟）比密集试验（ITI 为 1 分钟）支持更高的保留率，并且形成了依赖于蛋白质合成的晚期长时记忆（l-LTM）。我们的研究结果再次证实了嗅觉 SER 条件反射作为学习和记忆研究的可靠工具。
