Wüstenberg D, Gerber B, Menzel R
Institut für Neurobiologie, Freie Universität Berlin, K onigin-Luise-Str. 28/30, 14195 Berlin, Germany.
Eur J Neurosci. 1998 Aug;10(8):2742-5. doi: 10.1046/j.1460-9568.1998.00319.x.
Although work in a wide variety of species and paradigms has demonstrated that long-term memory is sensitive to the blocking of protein synthesis, previous studies have suggested that the honeybee might represent an exception to this rule. Retention tested one day after training was not impaired by the inhibition of translation by cycloheximide. Using blockers of either transcription (actinomycin D) or translation (anisomycin), we present experiments that reconcile this unusual finding by testing over longer retention periods. Honeybees were conditioned to associate an odourant with a sucrose reward. Typically, this leads to stable retention over days. However, injection of either drug led to lower retention after 4 days, whereas retention after 2 or sometimes even 3 days was unaffected. This dissociates two forms of memory: a protein synthesis-independent, medium-term memory (up to 3 days) and a protein synthesis-dependent, long-term memory lasting for at least 4 days.
尽管在各种物种和实验范式中的研究表明，长期记忆对蛋白质合成的阻断很敏感，但先前的研究表明蜜蜂可能是这一规则的例外。训练一天后进行的记忆保持测试并未因环己酰亚胺抑制翻译而受损。我们使用转录阻断剂（放线菌素D）或翻译阻断剂（茴香霉素）进行实验，通过在更长的记忆保持期进行测试来解释这一异常发现。蜜蜂被训练将一种气味与蔗糖奖励联系起来。通常情况下，这会导致数天的稳定记忆保持。然而，注射任何一种药物都会导致4天后记忆保持率降低，而2天甚至有时3天后的记忆保持不受影响。这区分了两种记忆形式：一种是不依赖蛋白质合成的中期记忆（长达3天）和一种依赖蛋白质合成的长期记忆，持续至少4天。
