体内毒性测试甲基蓝和苯胺蓝作为眼内手术的活体染料。
In vivo toxicity testing of methyl blue and aniline blue as vital dyes for intraocular surgery.
机构信息
Centre for Ophthalmology, University of Tuebingen, Tuebingen, Germany.
出版信息
Retina. 2009 Oct;29(9):1257-65. doi: 10.1097/IAE.0b013e3181b8615b.
PURPOSE
To investigate the biocompatibility of methyl blue and aniline blue as vital dyes for vitreoretinal surgery in an in vivo rat model and to evaluate the effect of these dyes on retinal structure and function.
METHODS
Adult Brown-Norway rats received intravitreal injections of 0.1%, 0.2%, and 2% methyl blue or aniline blue dissolved in balanced salt solution with balanced salt solution serving as a control. Retinal toxicity was assessed 7 days thereafter by means of retinal ganglion cell counts, light microscopy, and electroretinography.
RESULTS
No significant decrease in retinal ganglion cell counts at concentrations up to 0.2% was observed. At 2%, however, a significant retinal ganglion cell loss was detected with both dyes (more pronounced for aniline blue). Light microscopy showed no structural changes in the central retina for concentrations up to 0.2%. Electroretinographies detected no adverse effects of methyl blue or aniline blue on rod- or cone-driven responses at concentrations up to 0.2%.
CONCLUSION
Methyl blue and aniline blue are very biocompatible and may, therefore, be usable for intraocular surgery. Further testing with other animal models will be necessary to confirm this. The safety margin of methyl blue is possibly higher than that of aniline blue.
目的
在活体大鼠模型中研究亚甲蓝和苯胺蓝作为眼内视网膜手术中的活体染料的生物相容性，并评估这些染料对视网膜结构和功能的影响。
方法
成年 Brown-Norway 大鼠接受 0.1%、0.2%和 2%的亚甲蓝或苯胺蓝在平衡盐溶液中的玻璃体注射，以平衡盐溶液作为对照。7 天后通过视网膜神经节细胞计数、光镜和视网膜电图评估视网膜毒性。
结果
在浓度高达 0.2%时，没有观察到视网膜神经节细胞计数的显著下降。然而，在 2%时，两种染料都检测到明显的视网膜神经节细胞丢失（苯胺蓝更明显）。在浓度高达 0.2%时，光镜显示中央视网膜没有结构变化。视网膜电图检测到在浓度高达 0.2%时，亚甲蓝或苯胺蓝对杆状或锥状驱动反应没有不良影响。
结论
亚甲蓝和苯胺蓝具有非常好的生物相容性，因此可能可用于眼内手术。需要进一步用其他动物模型进行测试以确认这一点。亚甲蓝的安全裕度可能高于苯胺蓝。
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