一种用于内界膜剥离的新型菁染料的体内生物相容性
In vivo biocompatibility of a new cyanine dye for ILM peeling.
作者信息
Thaler S, Haritoglou C, Schuettauf F, Choragiewicz T, May C A, Gekeler F, Fischer M D, Langhals H, Schatz A
机构信息
Eye Clinic and Institute for Ophthalmic Research, Centre for Ophthalmology, University of Tübingen, Tübingen, Germany.
Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany.
出版信息
Eye (Lond). 2015 Mar;29(3):428-35. doi: 10.1038/eye.2014.295. Epub 2014 Dec 19.
PURPOSE
To investigate the biocompatibility of the new cyanine dye: 3,3'-Di-(4-sulfobutyl)-1,1,1',1'-tetramethyl-di-1H-benz[e]indocarbocyanine (DSS) as a vital dye for intraocular application in an in vivo rat model and to evaluate the effects of this dye on retinal structure and function.
METHODS
DSS at a concentration of 0.5% was applied via intravitreal injections to adult Brown Norway rats with BSS serving as a control. Retinal toxicity was assessed 7 days later by means of retinal ganglion cell (RGC) counts, light microscopy, optical coherence tomography (OCT), and electroretinography (ERG).
RESULTS
No significant decrease in RGC numbers was observed. No structural changes of the central retina were observed either in vivo (OCT) or under light microscopy. ERGs detected a temporary reduction of retinal function 7 days after injection; this was no longer evident 14 days after injection.
CONCLUSIONS
DSS showed good biocompatibility in a well-established experimental in vivo setting and may be usable for intraocular surgery as an alternative to other cyanine dyes. In contrast to indocyanine green, it additionally offers fluorescence in the visual spectrum. Further studies with other animal models are needed before translation into clinical application.
目的
研究新型花青染料3,3'-二-(4-磺丁基)-1,1,1',1'-四甲基二-1H-苯并[e]吲哚碳菁染料（DSS）作为眼内应用的活性染料在大鼠体内模型中的生物相容性，并评估该染料对视网膜结构和功能的影响。
方法
将浓度为0.5%的DSS经玻璃体腔注射到成年棕色挪威大鼠体内，以平衡盐溶液（BSS）作为对照。7天后，通过视网膜神经节细胞（RGC）计数、光学显微镜、光学相干断层扫描（OCT）和视网膜电图（ERG）评估视网膜毒性。
结果
未观察到RGC数量显著减少。在活体（OCT）或光学显微镜下均未观察到视网膜中央结构的变化。视网膜电图检测到注射后7天视网膜功能暂时下降；注射14天后这种情况不再明显。
结论
在成熟的体内实验环境中，DSS显示出良好的生物相容性，可作为其他花青染料的替代品用于眼内手术。与吲哚菁绿不同，它在可见光谱中还具有荧光。在转化为临床应用之前，需要用其他动物模型进行进一步研究。
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