Agriculture and Agri-Food Canada Research Centre, 5403 1st Ave. S., P.O. Box 3000, Lethbridge, AB T1J 4B1, Canada.
Can J Microbiol. 2009 Oct;55(10):1224-7. doi: 10.1139/w09-080.
The effect of storing bovine feces in Cary-Blair medium on the recovery of antimicrobial-resistant Escherichia coli was investigated. Feces from cattle at a research feedlot (n = 50) and at a commercial feedlot (n = 46) were processed immediately or after storage in Cary-Blair medium for 8 days at 5 degrees C. Total, ampicillin-resistant, and tetracycline-resistant E. coli were isolated. The number of total E. coli decreased slightly after storage (0.19 log units; p < 0.001), but storage of feces in Cary-Blair medium did not affect recovery of ampicillin- or tetracycline-resistant E. coli.
研究了将牛粪便储存在 Cary-Blair 培养基中对抗菌药物耐药大肠杆菌回收的影响。立即处理来自研究饲料场(n = 50)和商业饲料场(n = 46)的牛粪便,或在 5°C 下储存 Cary-Blair 培养基 8 天后处理。分离出总大肠杆菌、氨苄青霉素耐药大肠杆菌和四环素耐药大肠杆菌。储存后总大肠杆菌数量略有下降(0.19 个对数单位;p < 0.001),但粪便在 Cary-Blair 培养基中的储存并不影响氨苄青霉素或四环素耐药大肠杆菌的回收。