Dipartimento di Colture Arboree, Universita degli Studi di Torino, 10095, Grugliasco (Torino), Italy.
Genome. 2009 Oct;52(10):839-48. doi: 10.1139/g09-057.
There is a lack of published microsatellite data which characterizes Ribes spp. To address this, an initial study of simple sequence repeat (SSR) variation was undertaken in 41 cultivars belonging to four species of the genus Ribes to evaluate its genetic variability. The cultivars were collected in Piedmont, northwest Italy, together with one cultivar from Switzerland. Twenty SSRs were screened for amplification and polymorphism. Seven failed to amplify, and therefore the remaining 13 were selected and used to fingerprint all the cultivars. Microsatellite analysis resulted in the identification of 38 genotypes, suggesting the existence of possible clonal genotypes and synonyms. Among the cultivars analyzed, two tetraploid accessions were found. The evaluation of genetic variability in Ribes is of fundamental importance for future nutritional breeding programs and to preserve genetic resources, as cultivar characterization permits better management of plant collections.
有关 Ribes spp. 的发表的微卫星数据较少。为了解决这个问题，对来自 Ribes 属的四个物种的 41 个品种进行了简单序列重复（SSR）变异的初步研究，以评估其遗传变异性。这些品种与瑞士的一个品种一起在意大利西北部的皮埃蒙特收集。筛选了 20 个 SSR 进行扩增和多态性分析。其中 7 个未能扩增，因此选择了其余 13 个 SSR 并用于对所有品种进行指纹分析。微卫星分析鉴定出 38 种基因型，表明可能存在无性系基因型和同义词。在所分析的品种中，发现了两个四倍体品系。对 Ribes 遗传变异性的评估对于未来的营养育种计划和保护遗传资源非常重要，因为品种特征可以更好地管理植物收集。
