Department of Otorhinolaryngology - Head and Neck Surgery, Kanazawa University, Kahokugun, Ishikawa, Japan.
Rhinology. 2009 Dec;47(4):460-4. doi: 10.4193/Rhin08.213.
To image olfactory nerve regeneration in vivo using a high-resolution gamma cam- era and radiography after nasal administration of thallium-201 (olfacto-scintigraphy).
Six Wistar rats were trained to avoid the smell of cycloheximide as a test of olfactory function. The olfactory nerve fibers of 3 rats were then carefully transected bilaterally with a Teflon knife, avoiding damage to the olfactory bulbs. The remaining 3 rats underwent sham operations and were used as controls. Steel wires were implanted in the left olfactory bulb of each rat for locating the bulbs with plain X-rays. The rats were assessed 2, 14, 28, and 42 d after the olfactory nerve transection or sham operation for their ability to detect odours and for transport of 201Tl to the olfactory bulb area 8 h after nasal administration of 201Tl.
Both transport of 201Tl to the olfactory bulb area (p < 0.04) and ability to detect odours (p < 0.04) significantly increased with a time course after olfactory nerve transection.
201Tl transport to the olfactory bulb may be useful to visually assess olfactory ability in vivo. We plan to test olfacto-scintigraphy clinically by nasal administration of 201Tl in patients with posttraumatic olfactory loss.
通过鼻内给予铊-201（嗅闪烁扫描）后，使用高分辨率伽马相机和射线照相术在体成像嗅神经再生。
将 6 只 Wistar 大鼠训练成避免环已亚硝脲气味作为嗅觉功能的测试。然后，用聚四氟乙烯刀小心地双侧切断 3 只大鼠的嗅神经纤维，避免损伤嗅球。其余 3 只大鼠接受假手术，并用作对照。将钢丝植入每只大鼠的左侧嗅球中，用于通过普通 X 射线定位嗅球。在嗅神经切断或假手术后的 2、14、28 和 42 天，评估大鼠检测气味的能力以及 201Tl 在嗅神经切断后 8 小时经鼻内给予 201Tl 后向嗅球区的转运。
嗅神经切断后，201Tl 向嗅球区的转运（p < 0.04）和检测气味的能力（p < 0.04）均随时间推移而显著增加。
201Tl 向嗅球的转运可能有助于在体视觉评估嗅觉能力。我们计划通过鼻内给予 201Tl 对创伤后嗅觉丧失的患者进行嗅闪烁扫描的临床测试。
