Shiga Hideaki, Kinoshita Yayoi, Washiyama Kohshin, Ogawa Daisuke, Amano Ryohei, Hirota Kyoko, Tsukatani Toshiaki, Furukawa Mitsuru, Miwa Takaki
Department of Otorhinolaryngology, Graduate School of Medical Science, Kanazawa University, 13-1 Takaramachi, Kanazawa, Ishikawa 920, Japan.
Chem Senses. 2008 Sep;33(7):633-7. doi: 10.1093/chemse/bjn030. Epub 2008 Jun 4.
Although olfactory nerve damage is a contributing factor in the diagnosis of posttraumatic olfactory loss, at present, there are no methods to directly assess injury to these nerves. We have shown that following olfactory nerve injury in mice, thallium-201 (201 Tl) transport from the nasal cavity to the olfactory bulb decreases. To determine if olfactory function after nerve injury could be assessed with nasal administration of 201 Tl, we measured the correlation between odor detection ability (ODA) and the rate of transport of 201 Tl in olfactory nerves. Both ODA and 201 Tl transport were measured after bilateral olfactory nerve transection for a 4-week period. Cycloheximide solution was used for ODA against tap water. 201 Tl transport was measured as the ratio of radioactivity in the nasal cavity and olfactory bulb with gamma spectrometry. There was a significant correlation between ODA and the rate of 201 Tl transport in the olfactory nerve. These findings suggest that olfactory function after nerve injury can be objectively evaluated with the nasal administration of 201 Tl.
尽管嗅神经损伤是创伤后嗅觉丧失诊断中的一个促成因素，但目前尚无直接评估这些神经损伤的方法。我们已经表明，在小鼠嗅神经损伤后，铊-201（²⁰¹Tl）从鼻腔向嗅球的转运减少。为了确定经鼻腔给予²⁰¹Tl是否可以评估神经损伤后的嗅觉功能，我们测量了气味检测能力（ODA）与²⁰¹Tl在嗅神经中的转运速率之间的相关性。在双侧嗅神经横断4周后，同时测量了ODA和²⁰¹Tl转运。使用环己酰亚胺溶液针对自来水测量ODA。通过γ能谱法测量²⁰¹Tl转运，以鼻腔和嗅球中的放射性比值表示。ODA与²⁰¹Tl在嗅神经中的转运速率之间存在显著相关性。这些发现表明，经鼻腔给予²⁰¹Tl可以客观地评估神经损伤后的嗅觉功能。
