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[通过聚丙烯酰胺凝胶电泳研究劳斯肉瘤病毒(RSV)的细胞污染物和结构蛋白]

[Cellular contaminants and structural proteins of Rous sarcoma virus (RSV), studied by polyacrylamide gel electrophoresis].

作者信息

Connan G, Rabotti G F

出版信息

C R Acad Hebd Seances Acad Sci D. 1977 Sep 12;285(4):463-5.

PMID:199367
Abstract

The number of polypeptides in highly purified preparations of RSV, of two different subgroups, produced in culture, has been compared to the polypeptides present in the supernatant of uninfected cultures and processed in identical manner. The analysis of PAGE-SDS shows that from 13 to 18 polypeptides present in viral preparations may be cellular contaminants. Fewer contaminating polypeptides are found in the myeloblastosis virus purified from plasma of Chicken.

摘要

对在培养物中产生的两种不同亚组的高纯度呼吸道合胞病毒(RSV)制剂中的多肽数量,与未感染培养物上清液中以相同方式处理的多肽进行了比较。SDS-聚丙烯酰胺凝胶电泳(PAGE-SDS)分析表明,病毒制剂中存在的13至18种多肽可能是细胞污染物。从鸡血浆中纯化的成髓细胞瘤病毒中发现的污染性多肽较少。

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