The Lester and Sue Smith Breast Center and Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
J Mammary Gland Biol Neoplasia. 2009 Dec;14(4):411-5. doi: 10.1007/s10911-009-9155-3. Epub 2009 Nov 24.
Whole mount preparations of mouse mammary glands are useful for evaluating overall changes in growth and morphology, and are essential for detecting and evaluating focal or regionally-localized phenotypes that would be difficult to detect or analyze using other techniques. We present three newly developed methods for preparing whole mounts of mammary glands from genetically-engineered mice expressing fluorescent proteins, as well as using either neutral red or a variety of fluorescent dyes. Unlike traditional hematoxylin- or carmine-stained preparations, neutral red-stained and some fluorescent preparations can be used for several common downstream analyses.
制作全乳鼠乳腺标本对于评估生长和形态的整体变化非常有用，对于检测和评估局部或区域性的表型尤为重要，这些表型用其他技术很难检测或分析。我们介绍了三种新开发的方法，用于制备表达荧光蛋白的基因工程鼠的全乳鼠乳腺标本，同时还使用了中性红或多种荧光染料。与传统的苏木精或胭脂红染色的标本不同，中性红染色和一些荧光染色的标本可用于几种常见的下游分析。
