Panchal Heena, Wansbury Olivia, Howard Beatrice A
The Breakthrough Breast Cancer Research Centre, The Institute of Cancer Research, London, UK.
Methods Mol Biol. 2010;585:261-70. doi: 10.1007/978-1-60761-380-0_18.
The mouse mammary gland is a unique organ since although the mammary gland primordium forms during embryogenesis, the majority of development occurs postnatally upon hormonal stimulation at puberty and full functionality (i.e. lactation) is not achieved until after parturition. Since both the epidermis and mammary glands share the same developmental origin, many mouse models with epidermal phenotypes often also exhibit abnormal mammary gland development. However, since the most widely used methods to analyse mammary glands are laborious and time-consuming, many mouse models exist that have not been analysed for mammary phenotypes. We have developed a simplified method that allows rapid analysis of embryonic mammary anlagen using immunolabelling of whole mounts that should facilitate more comprehensive studies of mouse mammary glands.
小鼠乳腺是一个独特的器官，因为尽管乳腺原基在胚胎发育过程中形成，但大部分发育在出生后青春期受到激素刺激时发生，并且直到分娩后才实现完全功能（即泌乳）。由于表皮和乳腺具有相同的发育起源，许多具有表皮表型的小鼠模型通常也表现出乳腺发育异常。然而，由于分析乳腺最常用的方法既费力又耗时，许多小鼠模型尚未进行乳腺表型分析。我们开发了一种简化方法，可通过对整个标本进行免疫标记来快速分析胚胎乳腺原基，这应该有助于对小鼠乳腺进行更全面的研究。
