金刚烷胺对大鼠下肢缺血/再灌注损伤模型肺组织的影响。
The effects of amantadine on lung tissue in lower limb ischemia/reperfusion injury model in rats.
作者信息
Orhan Mustafa, Taş Tuna Ayça, Ünal Yusuf, Arslan Mustafa, Yazar Hayrullah, Sezen Şaban Cem, Gözükara Sezen Irmak, Palabıyık Onur
机构信息
Department of Anesthesiology of Reanimation, Sakarya University, Faculty of Medicine, Sakarya, Turkey.
Department of Anesthesiology of Reanimation, Gazi University, Faculty of Medicine, Ankara, Turkey.
出版信息
Turk Gogus Kalp Damar Cerrahisi Derg. 2021 Jan 13;29(1):77-83. doi: 10.5606/tgkdc.dergisi.2021.19884. eCollection 2021 Jan.
BACKGROUND
This study aims to evaluate the effect of amantadine on lung tissue of after lower limb ischemia/reperfusion injury in rats.
METHODS
A total of 24 Wistar rats were divided into four equal groups including six rats in each: sham group (Group S), amantadine group (Group A), ischemia/reperfusion group (Group I/R), and ischemia/reperfusion + amantadine group (Group I/R-A). All groups underwent a midline abdominal incision. In Groups I/R and I/R-A, the infrarenal abdominal aorta was clamped for 120 min and, then, reperfused for 120 min after removal of the clamp. Amantadine hydrochloride 45 mg/kg was administered intraperitoneally to the rats of Groups A and Group I/R-A 15 min before surgery. At the end of reperfusion period (240 min), all rats were sacrificed, and their lung tissues were obtained. Lung tissue catalase and superoxide dismutase activities and glutathione S-transferase and malondialdehyde levels were analyzed. Lung tissues were examined histopathologically.
RESULTS
Catalase activity was lower in Groups A, I/R, and I/R-A compared to Group S. Superoxide dismutase activity was higher in Group I/R than Group S. Superoxide dismutase activity in Groups I/R-A and A decreased, compared to Groups S and I/R. Glutathione S-transferase levels decreased in Groups I/R and A, compared to Group S. Glutathione S-transferase levels in Group I/R-A were higher than Groups I/R and A. The highest level of malondialdehyde was found in Group I/R and the lowest level was found in Group I/R-A. According to histopathological examination, infiltration scores were significantly lower in Group S than Groups I/R and I/R-A (p=0.009 and p=0.011, respectively). The alveolar wall thickening scores in Group I/R were also significantly higher than Groups S and Group A (p=0.001 and p=0.001, respectively).
CONCLUSION
Lung tissue can be affected histopathologically by ischemia/ reperfusion injury and this injury can be reversed by amantadine administration.
背景
本研究旨在评估金刚烷胺对大鼠下肢缺血/再灌注损伤后肺组织的影响。
方法
将24只Wistar大鼠平均分为四组，每组6只：假手术组（S组）、金刚烷胺组（A组）、缺血/再灌注组（I/R组）和缺血/再灌注+金刚烷胺组（I/R-A组）。所有组均行腹部正中切口。在I/R组和I/R-A组中，肾下腹主动脉夹闭120分钟，然后移除夹子后再灌注120分钟。在手术前15分钟，给A组和I/R-A组的大鼠腹腔注射45mg/kg盐酸金刚烷胺。在再灌注期结束时（240分钟），处死所有大鼠，获取其肺组织。分析肺组织过氧化氢酶和超氧化物歧化酶活性以及谷胱甘肽S-转移酶和丙二醛水平。对肺组织进行组织病理学检查。
结果
与S组相比，A组、I/R组和I/R-A组的过氧化氢酶活性较低。I/R组的超氧化物歧化酶活性高于S组。与S组和I/R组相比，I/R-A组和A组的超氧化物歧化酶活性降低。与S组相比，I/R组和A组的谷胱甘肽S-转移酶水平降低。I/R-A组的谷胱甘肽S-转移酶水平高于I/R组和A组。丙二醛水平在I/R组中最高，在I/R-A组中最低。根据组织病理学检查，S组的浸润评分显著低于I/R组和I/R-A组（分别为p=0.009和p=0.011）。I/R组的肺泡壁增厚评分也显著高于S组和A组（分别为p=0.001和p=0.001）。
结论
缺血/再灌注损伤可对肺组织造成组织病理学影响，而给予金刚烷胺可逆转这种损伤。
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