Centre for Neuroscience, University of Alberta, Edmonton, Alberta, Canada, T6G 2E9.
J Comp Neurol. 2010 Jan 10;518(2):175-98. doi: 10.1002/cne.22192.
Extensive research has revealed a fundamental organization of the cerebellum consisting of functional parasagittal zones. This compartmentalization has been well documented with respect to physiology, biochemical markers, and climbing fiber afferents. Less is known about the organization of mossy fiber afferents in general, and more specifically in relation to molecular markers such as zebrin. Zebrin is expressed by Purkinje cells that are distributed as a parasagittal array of immunopositive and immunonegative stripes. We examined the concordance of zebrin expression with visual mossy fiber afferents in the vestibulocerebellum (folium IXcd) of pigeons. Visual afferents project directly to folium IXcd as mossy fibers and indirectly as climbing fibers via the inferior olive. These projections arise from two retinal recipient nuclei: the lentiformis mesencephali (LM) and the nucleus of the basal optic root (nBOR). Although it has been shown that these two nuclei project to folium IXcd, the detailed organization of these projections has not been reported. We injected anterograde tracers into LM and nBOR to investigate the organization of mossy fiber terminals and subsequently related this organization to the zebrin antigenic map. We found a parasagittal organization of mossy fiber terminals in folium IXcd and observed a consistent relationship between mossy fiber organization and zebrin stripes: parasagittal clusters of mossy fiber terminals were concentrated in zebrin-immunopositive regions. We also describe the topography of projections from LM and nBOR to the inferior olive and relate these results to previous studies on the organization of climbing fibers and zebrin expression.
大量研究揭示了小脑的基本组织，由功能平行的区域组成。这种分区在生理学、生物化学标志物和 climbing fiber 传入方面都有很好的记录。关于 mossy fiber 传入的组织，一般来说了解较少，更具体地说，与 zebrin 等分子标志物的关系了解得更少。Zebrin 由 Purkinje 细胞表达，这些细胞呈免疫阳性和免疫阴性条纹的平行排列分布。我们研究了 zebrin 表达与鸽子前庭小脑（IXcd 叶）中视觉 mossy fiber 传入的一致性。视觉传入纤维直接投射到 IXcd 叶作为 mossy 纤维，间接通过下橄榄投射作为 climbing 纤维。这些投射来自两个视网膜接受核：中脑豆状核（LM）和基底视束核（nBOR）。虽然已经表明这两个核投射到 IXcd 叶，但这些投射的详细组织尚未报道。我们将顺行示踪剂注入 LM 和 nBOR，以研究 mossy fiber 末梢的组织，并将这种组织与 zebrin 抗原图谱相关联。我们在 IXcd 叶中发现了 mossy fiber 末梢的平行组织，并观察到 mossy fiber 组织和 zebrin 条纹之间存在一致的关系：mossy fiber 末梢的平行簇集中在 zebrin 免疫阳性区域。我们还描述了 LM 和 nBOR 到下橄榄的投射的拓扑结构，并将这些结果与以前关于 climbing fiber 和 zebrin 表达的组织的研究联系起来。
