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猪源脂蛋白-X的分离与鉴定

Isolation and characterization of lipoprotein-X from the pig.

作者信息

Müller P, Baggio G, Barth H O, Wieland H, Anari M, Seidel D

出版信息

Clin Chim Acta. 1977 Nov 1;80(3):487-94. doi: 10.1016/0009-8981(77)90142-5.

DOI:10.1016/0009-8981(77)90142-5
PMID:199382
Abstract

In vitro incubation of pig bile with pig whole serum resulted in the formation of "LP-X like" material migrating towards the cathode on agar-gel electrophoresis. As in all other species studied so far, LP-X occurred also in the serum of bile duct ligated pigs, however, relatively late (48-72h) after the operation. In parallel, alterations of some serum parameters and of the protein-lipid composition of the different lipoporotein fractions were observed. While the electrophoretic behaviour, the protein-lipid and apoprotein composition of pig LP-X were comparable to those found in man, dog and rat, it sedimated in most instances at the density 1.063 g/ml.

摘要

猪胆汁与猪全血清在体外孵育,在琼脂凝胶电泳上产生了向阴极迁移的“类LP-X”物质。与迄今研究的所有其他物种一样,结扎胆管的猪血清中也出现了LP-X,不过是在手术后相对较晚的时候(48 - 72小时)出现。同时,观察到一些血清参数以及不同脂蛋白组分的蛋白质 - 脂质组成发生了变化。虽然猪LP-X的电泳行为、蛋白质 - 脂质和载脂蛋白组成与人、狗和大鼠的相似,但在大多数情况下其密度为1.063 g/ml。

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