Hogquist K A, Nett M A, Sheehan K C, Pendleton K D, Schreiber R D, Chaplin D D
Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110.
J Immunol. 1991 Mar 1;146(5):1534-40.
The role of murine IL-1 beta in vitro and in vivo has not been defined. We describe here the production of neutralizing and immunoprecipitating mAb and polyclonal antibodies specific for murine IL-1 beta and their application to a characterization of the murine IL-1 beta protein. Immunization of either hamsters or rabbits with the recombinant mature form of murine IL-1 beta emulsified in CFA elicited antisera and hamster mAb that only recognized denatured IL-1 beta. In contrast, immunization with rIL-1 beta adsorbed to alum resulted in the generation of neutralizing and immunoprecipitating rabbit and hamster antisera and hamster mAb. All of the mAb recognize both the pro-form of IL-1 beta and the mature bioactive form produced by cultures of murine peritoneal macrophages. Using these antibodies, we demonstrate that approximately half of the IL-1 activity present in supernatants of LPS-treated cultured mouse macrophages is composed of IL-1 beta. Additionally, IL-1 beta as well as IL-1 alpha can be detected in the plasma of LPS-treated mice. These studies, therefore, demonstrate the production of IL-1 beta both in vitro and in vivo.
小鼠白细胞介素-1β在体外和体内的作用尚未明确。我们在此描述了针对小鼠白细胞介素-1β的中和性及免疫沉淀性单克隆抗体和多克隆抗体的产生，以及它们在小鼠白细胞介素-1β蛋白特性鉴定中的应用。用在弗氏完全佐剂中乳化的重组成熟形式小鼠白细胞介素-1β免疫仓鼠或兔子，可引发仅识别变性白细胞介素-1β的抗血清和仓鼠单克隆抗体。相比之下，用吸附于明矾的重组白细胞介素-1β免疫则导致产生中和性及免疫沉淀性兔和仓鼠抗血清以及仓鼠单克隆抗体。所有单克隆抗体均识别白细胞介素-1β的前体形式以及由小鼠腹腔巨噬细胞培养物产生的成熟生物活性形式。利用这些抗体，我们证明脂多糖处理的培养小鼠巨噬细胞上清液中约一半的白细胞介素-1活性由白细胞介素-1β组成。此外，在脂多糖处理的小鼠血浆中可检测到白细胞介素-1β以及白细胞介素-1α。因此，这些研究证明了白细胞介素-1β在体外和体内均有产生。
