Fuhlbrigge R C, Sheehan K C, Schreiber R D, Chaplin D D, Unanue E R
Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110.
J Immunol. 1988 Oct 15;141(8):2643-50.
We report the production of hamster anti-murine IL-1 alpha mAb and analysis of their specificity and suitability for use in murine IL-1 immunologic and biologic assays. The mAb bound to murine (Mu) rIL-1 alpha 3 but not rMuIL-1 beta as assessed by both direct ELISA and immunoprecipitation. They also inhibited the biologic activity of MuIL-1 alpha but not the activity of rMuIL-1 beta as measured in a T cell co-stimulator assay. These IL-1 alpha specific mAb only partially inhibited the co-stimulator activity found in macrophage culture supernatants but completely inhibited the co-stimulator activity of fixed peritoneal exudate cells. The data indicate that the species of IL-1 associated with murine macrophage membranes shares at least two epitopes with IL-1 alpha and probably represents a product of the IL-1 alpha gene. These reagents will be valuable for quantitative assessment of specific IL-1 proteins on cell surfaces, in culture supernatants, and in cell lysates. They will also be useful both in vitro and in vivo for determining the relative roles of the different IL-1 species in the development of biologic responses.
我们报告了仓鼠抗小鼠IL-1α单克隆抗体的制备及其特异性分析，以及它们在小鼠IL-1免疫和生物学检测中的适用性。通过直接ELISA和免疫沉淀评估，该单克隆抗体与小鼠（Mu）重组IL-1α3结合，但不与重组小鼠IL-1β结合。在T细胞共刺激试验中，它们还抑制了MuIL-1α的生物学活性，但不抑制重组小鼠IL-1β的活性。这些IL-1α特异性单克隆抗体仅部分抑制巨噬细胞培养上清液中的共刺激活性，但完全抑制固定腹膜渗出细胞的共刺激活性。数据表明，与小鼠巨噬细胞膜相关的IL-1种类与IL-1α至少共享两个表位，可能代表IL-1α基因的产物。这些试剂对于定量评估细胞表面、培养上清液和细胞裂解物中特定IL-1蛋白将是有价值的。它们在体外和体内对于确定不同IL-1种类在生物学反应发展中的相对作用也将是有用的。
