Urbaitis B K, Razynska A, Corteza Q, Fronticelli C, Bucci E
Department of Medicine (Nephrology Division), Medical School, University of Maryland, Baltimore 21201.
J Lab Clin Med. 1991 Feb;117(2):115-21.
Plasma half time of unmodified hemoglobin (UHb) and two intramolecularly cross-linked hemoglobins (alpha alpha XL and beta beta XL) was measured in anesthetized rats after an intravenous bolus of 20 mg.100 gm.-1 To rule out the possibility that differences among plasma half times might be caused by differences in acute effects on renal excretory function, glomerular filtration rate (GFR) and effective renal plasma flow (ERPF) were measured simultaneously with plasma half time. Experiments were also done to determine whether higher doses (60 to 100 mg-1.100 gm-1) of these compounds had a delayed effect (48 hours) on GFR or ERPF. Massive urinary excretion of UHb occurred; however, only 1% of the alpha alpha XL and none of the beta beta XL was excreted. Plasma half time of alpha alpha XL and beta beta XL averaged 3.3 hours, or four times longer than UHb. In no case did a decrease in GFR or ERPF occur. Instead, a transient increase in GFR, ERPF, urine flow, and systemic blood pressure was seen. Similar increases occurred after albumin administration, suggesting expansion of vascular volume as the initiating factor. Renal functions at 48 hours after 60 to 100 mg.100 gm-1 of UHb, alpha alpha XL or beta beta XL were not different from control (albumin). Intratubular hemoglobin casts or intravascular precipitates were not evident in acute or 48-hour studies. At 48 hours Perls' staining material was found in one alpha alpha XL specimen at 3 hours after administration. Perls' staining material was present in renal tubule cells in all but the albumin-treated kidneys.(ABSTRACT TRUNCATED AT 250 WORDS)
在给麻醉大鼠静脉推注20 mg·100 gm⁻¹未修饰血红蛋白（UHb）以及两种分子内交联血红蛋白（ααXL和ββXL）后，测定其血浆半衰期。为排除血浆半衰期差异可能由对肾排泄功能的急性影响不同所导致的可能性，在测定血浆半衰期的同时，还测定了肾小球滤过率（GFR）和有效肾血浆流量（ERPF）。还进行了实验，以确定这些化合物的较高剂量（60至100 mg·100 gm⁻¹）是否对GFR或ERPF有延迟效应（48小时）。出现了大量UHb经尿液排泄的情况；然而，仅1%的ααXL经尿液排泄，而ββXL则无经尿液排泄。ααXL和ββXL的血浆半衰期平均为3.3小时，是UHb的四倍。在任何情况下，GFR或ERPF均未降低。相反，观察到GFR、ERPF、尿流量和全身血压出现短暂升高。输注白蛋白后也出现了类似的升高，提示血管容量扩张是起始因素。给予60至100 mg·100 gm⁻¹的UHb、ααXL或ββXL后48小时的肾功能与对照组（白蛋白）无异。在急性或48小时研究中，未发现肾小管内血红蛋白管型或血管内沉淀物。在给药后3小时，在一个ααXL标本中于48小时发现了Perls染色物质。除白蛋白处理的肾脏外，在所有肾脏的肾小管细胞中均存在Perls染色物质。（摘要截短于250字）
