Yan Fengxia, Wang Kangcai, Luo Qingyun, Luo Chunhong
Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China.
Zhongguo Zhong Yao Za Zhi. 2009 Aug;34(16):2039-42.
To study the effect of NH4(+)-N/NO3(-)-N ratios in the applied supplementary fertilizer on the growth, nitrogen metabolis related enzymes activity and photosynthetic characteristics of Isatis indigotica.
The sand culture experiment was conducted, and seedling of I. indigotica was fertilized with the mixed nutrition that containing the Hoagland's macro elements and the Aron's micro elements, the additional 63 mmol N was supplementary with the NH4(+)-N/NO3(-)-N ratio of 100:0, 75:25, 50:50, 25:75 and 0:100.
The biomass of I. indigotica increased at first when the supplementary N of NH4(+)-N/NO3(-)-N ratio changed from 100:0 to 50:50 and decreased afterwards. The maximum value was at 50:50 and the minimum at 100: 0. With increasing the ratio of NO3(-)-N, the activity of nitrate reductase and glutamine synthetase increased and then decreased and the relationship between the activity and the ratio could be described with an approximate parabola curve. The net photosynthetic rate of I. indigotica was the highest at the NH4(+)-N/NO3(-)-N ratio of 75:25 and the lowest at 100:0.
Increasing the NO3(-)-N ratio properly was beneficial to promote the growth and improve the activity of nitrate reductase and glutamine synthetase and net photosynthetic rate of I. indigotica.
研究追肥中铵态氮/硝态氮比例对菘蓝生长、氮代谢相关酶活性及光合特性的影响。
采用砂培试验，用含有霍格兰大量元素和阿隆微量元素的混合营养液对菘蓝幼苗施肥，额外补充63 mmol氮，铵态氮/硝态氮比例分别为100:0、75:25、50:50、25:75和0:100。
随着铵态氮/硝态氮比例从100:0变化到50:50，菘蓝生物量先增加后降低，在50:50时最大，100:0时最小。随着硝态氮比例增加，硝酸还原酶和谷氨酰胺合成酶活性先升高后降低，二者与比例的关系可用近似抛物线描述。菘蓝净光合速率在铵态氮/硝态氮比例为75:25时最高，100:0时最低。
适当提高硝态氮比例有利于促进菘蓝生长，提高硝酸还原酶、谷氨酰胺合成酶活性及净光合速率。
