两种线虫传多面体病毒（番茄黑环病毒和葡萄铬花叶病毒）的RNA - 2编码多聚蛋白的体外加工

In vitro processing of the RNA-2-encoded polyprotein of two nepoviruses: tomato black ring virus and grapevine chrome mosaic virus.

作者信息

Demangeat G, Hemmer O, Fritsch C, Le Gall O, Candresse T

机构信息

Institut de Biologie Moléculaire des Plantes du CNRS, Université Louis Pasteur, Strasbourg, France.

出版信息

J Gen Virol. 1991 Feb;72 ( Pt 2):247-52. doi: 10.1099/0022-1317-72-2-247.

DOI:10.1099/0022-1317-72-2-247

PMID:1993868

Abstract

In vitro translation of RNA-2 of each of two closely related nepoviruses, tomato black ring virus (TBRV) and grapevine chrome mosaic virus (GCMV), in a rabbit reticulocyte lysate resulted in the synthesis of single polypeptides of 150K and 146K respectively. Processing of these polyproteins occurred after the addition of translation products of homologous RNA-1. The positions of the cleavage products within the polyproteins were determined. From the N to the C terminus, Mr values for the proteins were 50K, 46K and 59K for TBRV and 44K, 46K and 56K for GCMV. TBRV RNA-1 translation products also cleaved the polyproteins encoded by GCMV RNA-2 which suggests that the cleavage sites in the two polyproteins are similar.

摘要

在兔网织红细胞裂解物中对两种密切相关的线虫传多面体病毒——番茄黑环病毒（TBRV）和葡萄铬花叶病毒（GCMV）的RNA - 2进行体外翻译，分别产生了150K和146K的单一多肽。这些多聚蛋白的加工在加入同源RNA - 1的翻译产物后发生。确定了多聚蛋白内裂解产物的位置。从N端到C端，TBRV的蛋白质Mr值分别为50K、46K和59K，GCMV的为44K、46K和56K。TBRV RNA - 1的翻译产物也能裂解由GCMV RNA - 2编码的多聚蛋白，这表明两种多聚蛋白中的裂解位点相似。