Bu M, Shih D S
Department of Biochemistry, Louisiana State University, Baton Rouge.
Virology. 1989 Nov;173(1):348-51. doi: 10.1016/0042-6822(89)90255-9.
Cleavages of the polyproteins synthesized from cowpea mosaic virus (CPMV) B RNA and M RNA in rabbit reticulocyte lysates are inhibited by hemin. Cleavage of the CPMV B RNA-encoded 200K polyprotein and of the M RNA-encoded 60K intermediary precursor protein were most sensitive to hemin inhibition, while cleavages of other precursor proteins were less sensitive. A significant observation was that at a hemin concentration of 25 microM, but not at higher concentrations, the 60K protein was cleaved to yield the two viral capsid proteins. This cleavage reaction has not been observed in vitro previously.
在兔网织红细胞裂解物中，由豇豆花叶病毒（CPMV）B RNA和M RNA合成的多聚蛋白的切割受到血红素的抑制。CPMV B RNA编码的200K多聚蛋白和M RNA编码的60K中间前体蛋白的切割对血红素抑制最为敏感，而其他前体蛋白的切割则不太敏感。一个重要的发现是，在血红素浓度为25微摩尔时，而非更高浓度时，60K蛋白被切割产生两种病毒衣壳蛋白。此前在体外尚未观察到这种切割反应。
