Acosta O, Mayo M A
Scottish Crop Research Institute, Invergowrie, Dundee, Scotland.
Arch Virol. 1993;129(1-4):145-54. doi: 10.1007/BF01316891.
Infection of Nicotiana clevelandii protoplasts by raspberry ringspot nepovirus resulted in the accumulation of about 24 polypeptides that differed in M(r) and pI from polypeptides accumulating in mock-inoculated protoplasts. Similar polypeptides accumulated in protoplasts infected with the S and E strains of RRV but different infection-specific polypeptides were detected in protoplasts infected with tobacco ringspot nepovirus. The M(r) of RRV-specific polypeptides ranged from 210,000 to 18,000 and most are presumed to be derived from others by proteolytic cleavage. No evidence was found for marked changes in polypeptide abundance with time after inoculation or for any virus-specific polypeptide becoming disproportionately abundant in the medium during culture.
悬钩子环斑线虫传多面体病毒感染克利夫兰烟草原生质体后，积累了约24种多肽，其相对分子质量（M(r)）和等电点（pI）与模拟接种原生质体中积累的多肽不同。用悬钩子环斑病毒（RRV）的S株和E株感染原生质体时也积累了类似的多肽，但在用烟草环斑线虫传多面体病毒感染的原生质体中检测到了不同的感染特异性多肽。RRV特异性多肽的相对分子质量范围为210,000至18,000，大多数被认为是通过蛋白水解切割从其他多肽衍生而来。没有发现接种后多肽丰度随时间有明显变化的证据，也没有发现任何病毒特异性多肽在培养过程中在培养基中变得异常丰富的证据。
