Department of Genome Sciences, University of Washington, Seattle, Washington 98195, USA.
J Proteome Res. 2010 Feb 5;9(2):1138-43. doi: 10.1021/pr900816a.
In shotgun proteomics, the analysis of tandem mass spectrometry data from peptides can benefit greatly from high mass accuracy measurements. In this study, we have evaluated two database search strategies which use high mass accuracy measurements of the peptide precursor ion. Our results indicate that peptide identifications are improved when spectra are searched with a wide mass tolerance window and precursor mass is used as a filter to discard incorrect matches. Database searches with a peptide data set constrained to peptides within a narrow mass window resulted in fewer peptide identifications but a significantly faster database search time.
在鸟枪法蛋白质组学中，对肽段的串联质谱数据进行分析可以从高质量精度测量中获益匪浅。在这项研究中，我们评估了两种数据库搜索策略，这些策略使用肽前体离子的高质量精度测量。我们的结果表明，当使用较宽的质量容限窗口搜索谱图并使用前体质量作为过滤条件来排除错误匹配时，可以提高肽的鉴定率。使用质量窗口较窄的肽数据集对数据库进行搜索，虽然鉴定到的肽数量较少，但数据库搜索时间明显缩短。
