Phase behavior of Phytantriol/water bicontinuous cubic Pn3m cubosomes stabilized by Laponite disc-like particles.

The present article reports on the specific effects of temperature on Phytantriol-based cubosomes stabilized by inorganic stabilizers as opposed to organic stabilizers. The ability of Laponite to stabilize Phytantriol-based parent bulk phase is first demonstrated. The sub-micron-sized Laponite-stabilized particles were found to be both physically and chemically stable over time. The temperature-induced behavior, both in heating and cooling directions, of these lipid-based cubosomes has been investigated and compared with their polymer-stabilized counterparts (Pluronic F127). This allows us to extract the particular influence of each stabilizer. Whereas an increased hydration of the cubic structure was evidenced at high pH values, this effect was eliminated to compare the specific influence of both stabilizers on these Phytantriol-based cubosomes. Evidence of differences in the relaxation rates of the internal structures with temperature was found for the two stabilizers, in particular in the cooling direction whilst in the heating direction the two stabilizers could be considered as undisruptive. The origin of this difference is discussed.