Turkish Academy of Sciences, Piyade Sokak No. 27, 06550 Cankaya, Ankara, Turkey.
Bioresour Technol. 2010 Apr;101(8):2665-71. doi: 10.1016/j.biortech.2009.09.094. Epub 2009 Nov 24.
This study investigated the fate of 2,6-dihydroxybenzoic acid in a mixed microbial culture acclimated to peptone under aerobic conditions. A laboratory-scale sequencing batch reactor receiving a pulse feeding of peptone at the start of each daily cycle was used for this purpose. Experimental evaluations interpreted changes induced by continuous benzoic acid additions on the oxygen uptake rate profiles associated with peptone biodegradation. At first exposure, 2,6-dihydroxybenzoic acid reduced the activity of the mixed culture and impaired peptone biodegradation. Around one-third of peptone removed could be utilized for microbial metabolism. With continuous feeding the mixture culture became acclimated and simultaneously removed peptone and 2,6-dihydroxybenzoic acid. After 30 days, oxygen uptake rate tests performed separately on peptone, 2,6-dihydroxybenzoic acid and the substrate mixture supported the existence of a dual biomass restructured with the selective growth of another group of microorganisms capable of utilizing 2,6-dihydroxybenzoic acid as an organic carbon source.
本研究考察了 2,6-二羟基苯甲酸在有氧条件下、适应于蛋白胨的混合微生物培养物中的命运。为此，使用了一个实验室规模的序批式反应器，在每个每日周期开始时接受蛋白胨的脉冲进料。实验评估解释了连续添加苯甲酸对与蛋白胨生物降解相关的耗氧率曲线所引起的变化。初次暴露时，2,6-二羟基苯甲酸会降低混合培养物的活性并损害蛋白胨的生物降解。约三分之一去除的蛋白胨可用于微生物代谢。随着连续进料，混合培养物逐渐适应，同时去除蛋白胨和 2,6-二羟基苯甲酸。30 天后，分别对蛋白胨、2,6-二羟基苯甲酸和基质混合物进行的耗氧率测试支持了存在一种双生物质的假设，其中另一组能够将 2,6-二羟基苯甲酸用作有机碳源的微生物选择性生长，从而重构了生物质。
