Department of Environmental Engineering, Faculty of Engineering and Architect, Campus of Avsar, Kahramanmaraş Sütçü Imam University, 46100 Kahramanmaraş, Turkey.
Environ Sci Pollut Res Int. 2012 Sep;19(8):3412-20. doi: 10.1007/s11356-012-0866-4. Epub 2012 Apr 15.
This study investigated the acute effect of benzo[a]anthracene, a significant compound among polycyclic aromatic hydrocarbons, on the biodegradation of a synthetic organic substrate-a peptone/meat extract mixture-under aerobic conditions.
A laboratory-scale sequencing batch reactor was sustained at steady state at a sludge age of 10 days with substrate feeding. Inhibition tests involved running a series of batch reactors initially seeded with the biomass obtained from the parent reactor. After the biomass seeding, the reactors were started with the peptone mixture and a range of initial benzo[a]anthracene concentrations between 0.5 and 88 mg/L. Experimental profiles of oxygen uptake rates and polyhydroxyalkanoates were evaluated by calibration of a selected model.
Lower doses of benzo[a]anthracene had no effect on process kinetics. The noticeable acute impact was only observed with the addition of 88 mg/L of benzo[a]anthracene, but it was limited with the storage mechanism: the amount of organic substrate diverted to polyhydroxyalkanoates was significantly reduced with a corresponding decrease in the maximum storage rate, k (STO), from 2.7 down to 0.6 day(-1). Similarly, the maximum growth rate from internally stored polyhydroxyalkanoates was lowered from 2.3 to 1.0 day(-1).
Among the mechanisms for direct substrate utilization, only the hydrolysis rate was slightly reduced, but otherwise, the overall COD removal efficiency was not affected.
本研究考察了苯并[a]蒽（多环芳烃中的一种重要化合物）对有氧条件下一种合成有机基质（蛋白胨/肉浸液混合物）生物降解的急性影响。
在稳定的实验室规模序批式反应器中，以 10 天的污泥龄和基质进料维持稳定状态。抑制试验涉及运行一系列批式反应器，最初用从母体反应器获得的生物质接种。在生物量接种后，使用蛋白胨混合物和 0.5 至 88mg/L 之间的一系列初始苯并[a]蒽浓度启动反应器。通过选择模型的校准评估耗氧率和聚羟基烷酸酯的实验曲线。
较低剂量的苯并[a]蒽对过程动力学没有影响。只有添加 88mg/L 的苯并[a]蒽时才会观察到明显的急性影响，但受到存储机制的限制：有机基质转向聚羟基烷酸酯的量显著减少，最大存储速率 k（STO）从 2.7 降至 0.6 天（-1）。同样，从内部储存的聚羟基烷酸酯中获得的最大生长速率从 2.3 降至 1.0 天（-1）。
在直接利用基质的机制中，只有水解速率略有降低，但总的 COD 去除效率不受影响。
