Environmental Engineering Department, Faculty of Civil Engineering, Istanbul Technical University, 34469 Maslak, Istanbul, Turkey.
Bioresour Technol. 2011 Jan;102(2):567-75. doi: 10.1016/j.biortech.2010.07.112. Epub 2010 Aug 1.
This study evaluated the kinetics of simultaneous biodegradation of peptone mixture and 2,6-dihydroxybenzoic acid (2,6-DHBA) by an acclimated dual microbial culture under aerobic conditions. A laboratory-scale sequencing batch reactor was sustained at steady-state with peptone mixture feeding. During the study period, peptone mixture feeding was continuously supplemented with 2,6-DHBA. Related experimental data were derived from three sets of parallel batch reactors, the first fed with the peptone mixture, the second with 2,6-DHBA and the third one with the two substrates, after acclimation of microbial culture and simultaneous biodegradation of both organics. A mechanistic model was developed for this purpose including the necessary model components and process kinetics for the model calibration of relevant experimental data. Model evaluation provided all biodegradation characteristics and kinetics for both peptone mixture and 2,6-DHBA. It also supported the development of a dual microbial community through acclimation, with the selective growth of a second group of microorganisms specifically capable of metabolizing 2,6-DHBA as an organic carbon source.
本研究评估了在有氧条件下，驯化的双微生物培养物同时生物降解蛋白胨混合物和 2,6-二羟基苯甲酸（2,6-DHBA）的动力学。实验室规模的序批式反应器在持续进料蛋白胨混合物的条件下维持稳定状态。在研究期间，连续向 2,6-DHBA 中补充蛋白胨混合物。相关实验数据来自三组平行的分批式反应器，第一个仅用蛋白胨混合物进料，第二个仅用 2,6-DHBA 进料，第三个同时用两种底物进料，在驯化微生物培养物和同时生物降解两种有机物之后。为此目的开发了一个机理模型，该模型包括必要的模型组件和过程动力学，用于相关实验数据的模型校准。模型评估提供了两种有机物，即蛋白胨混合物和 2,6-DHBA 的所有生物降解特征和动力学。它还支持通过驯化开发出双微生物群落，选择性地生长出第二组微生物，这些微生物专门能够将 2,6-DHBA 作为有机碳源进行代谢。
