State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Dec 15;877(32):4159-63. doi: 10.1016/j.jchromb.2009.11.001. Epub 2009 Nov 10.
A novel rapid chromatographic method based on utilization of UPLC column was developed for the analysis of eight active compounds in silymarin. The analysis was performed on a Waters Acquity UPLC system with an Acquity UPLC(BEH) C18 column (5mmx2.1mm I.D., 1.7mum) and a gradient elution of methanol and water containing 0.01% formic acid with a run time of 9min, in which the retention time of the last analyte was 5.8min. And all eight active compounds achieved complete separation. Comparison of system performance with conventional HPLC was made with respect to analysis time, efficiency and sensitivity. The results indicated that the type of column, the type of mobile phase and the modified addition were significant to the separation of isomeric compounds in herb extracts.
建立了一种基于 UPLC 柱利用的新型快速色谱法，用于分析水飞蓟素中八种活性化合物。分析在 Waters Acquity UPLC 系统上进行，采用 Acquity UPLC(BEH)C18 柱（5mmx2.1mm ID，1.7μm），甲醇和含 0.01%甲酸的水进行梯度洗脱，运行时间为 9min，最后一个分析物的保留时间为 5.8min。所有八种活性化合物均实现完全分离。就分析时间、效率和灵敏度而言，对常规 HPLC 与系统性能进行了比较。结果表明，柱类型、流动相类型和改性添加剂对草药提取物中异构体化合物的分离具有重要意义。
