Division of Enteric and Hepatitis Viruses, Center for Infectious Diseases, National Institute of Health, 5 Nokbun-dong, Eunpyung-gu, Seoul 122-701, South Korea.
J Clin Microbiol. 2010 Feb;48(2):615-6. doi: 10.1128/JCM.01790-09. Epub 2009 Nov 25.
To increase detection sensitivity, we modified primers using complementary locked primer (CLP) technology. The sensitivity of the reverse transcription-PCR (RT-PCR) with CLP-modified primers was 10- to 100-fold higher than that of RT-PCR without these primers. CLP-modified primers can increase sensitivity, providing a widely accessible method for molecular diagnosis.
为了提高检测灵敏度,我们使用互补锁定引物 (CLP) 技术对引物进行了修饰。使用 CLP 修饰引物的逆转录-PCR (RT-PCR) 的灵敏度比没有这些引物的 RT-PCR 高 10-100 倍。CLP 修饰的引物可以提高灵敏度,为分子诊断提供了一种广泛适用的方法。