Iha Koichiro, Omatsu Tsutomu, Watanabe Shumpei, Ueda Naoya, Taniguchi Satoshi, Fujii Hikaru, Ishii Yoshiyuki, Kyuwa Shigeru, Akashi Hiroomi, Yoshikawa Yasuhiro
Department of Biomedical Science, Graduate School of Agriculture and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
J Vet Med Sci. 2010 Feb;72(2):217-20. doi: 10.1292/jvms.09-0050. Epub 2009 Nov 25.
In this study, cDNA of Toll-like receptors (TLR) 3, 7 and 9 were synthesized and completely sequenced. The coding regions of cDNA for bat TLR3, TLR7 and TLR9 were 2,718, 3,150 and 3,090 bp in length, respectively. The open reading frames encoded 905, 1,049 and 1,029 amino acids for TLR3, TLR7 and TLR9, respectively. The nucleotide sequences, predicted amino acid sequences and predicted domain structures of the three bat TLRs had high homology with those of other mammals. In addition, the expression profiles of each TLR in main organs were analyzed. Expression of TLR3 was highest in the liver, whereas the expressions of TLR7 and TLR9 were highest in the spleen.
在本研究中，合成了Toll样受体（TLR）3、7和9的cDNA并进行了全序列测定。蝙蝠TLR3、TLR7和TLR9的cDNA编码区长度分别为2718、3150和3090 bp。开放阅读框分别为TLR3、TLR7和TLR9编码905、1049和1029个氨基酸。三种蝙蝠TLR的核苷酸序列、预测的氨基酸序列和预测的结构域结构与其他哺乳动物的具有高度同源性。此外，还分析了每个TLR在主要器官中的表达谱。TLR3在肝脏中的表达最高，而TLR7和TLR9在脾脏中的表达最高。
