Jakubzick Claudia, Randolph Gwendalyn J
Department of Gene and Cell Medicine, Icahn Research Institute, Mount Sinai School of Medicine, New York, NY, USA.
Methods Mol Biol. 2010;595:371-82. doi: 10.1007/978-1-60761-421-0_24.
Dendritic cell migration from the airway to the lymph nodes is a key event in the development of airway immunity during infection, allergy, and vaccination. With judicial selection of materials, there are two approaches to study dendritic cell migration to the mediastinal lymph nodes without the induction of inflammation: airway administration of fluorescent OVA and latex beads. Our protocol describes how to label and track pulmonary dendritic cells from the airway and lung to the mediastinal lymph nodes and reveals how to avoid pitfalls and suboptimal assays.
在感染、过敏和疫苗接种过程中，树突状细胞从气道迁移至淋巴结是气道免疫发展中的关键事件。通过合理选择材料，有两种方法可用于在不引发炎症的情况下研究树突状细胞向纵隔淋巴结的迁移：经气道给予荧光卵清蛋白（OVA）和乳胶珠。我们的方案描述了如何标记和追踪从气道及肺部至纵隔淋巴结的肺树突状细胞，并揭示了如何避免陷阱和不理想的检测方法。
